Knight:Materials: Difference between revisions
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*[http://www.piercenet.com/products/browse.cfm?fldID=02020108 Albumin standard] from Pierce | *[http://www.piercenet.com/products/browse.cfm?fldID=02020108 Albumin standard] from Pierce | ||
*[http://www.invitrogen.com/content/sfs/manuals/invisionstain_man.pdf InVision™ His-tag In-gel Stain] from Invitrogen (purchased in 2003?) | *[http://www.invitrogen.com/content/sfs/manuals/invisionstain_man.pdf InVision™ His-tag In-gel Stain] from Invitrogen (purchased in 2003?) | ||
==Dry ice== | ==Dry ice== | ||
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Dry ice is delivered on Tuesday mornings (before noon). The stash usually lasts all week but is gone some time over the weekend. There's not any remaining by Monday morning. | Dry ice is delivered on Tuesday mornings (before noon). The stash usually lasts all week but is gone some time over the weekend. There's not any remaining by Monday morning. | ||
== | ==Stocks== | ||
*[[Knight:Freezer]] | |||
*[[Knight:yeast plasmids|Plasmids]] | |||
*[[Knight:yeast strains|Strains]] | |||
[[ | ==Quartz cuvettes== | ||
For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes. I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the [http://csbi.mit.edu/biophysinstrument/cdspectrometer CD spectrometer] at the [http://csbi.mit.edu/biophysinstrument/ Bioinstrumentation facility]. The two microcell cuvettes were not suitable for CD ... they have high birefringence. The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm. However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm. So these cuvettes are not great for this purpose. | |||
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Revision as of 14:27, 7 October 2006
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Knight Lab
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Common primers
VF2
5' tgc cac ctg acg tct aag aa 3'
VR
5' ATT ACC GCC TTT GAG TGA GC 3'
BioBricks-f
5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
- ordered by Reshma 5/1/2006 (25μM)
BioBricks-r
5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
- ordered by Reshma 8/24/2006
M13-R
5' cag gaa aca gct atg ac 3'
- ordered by Austin (50μM)
M13-F (-20)
5' GTA AAA CGA CGG CCA G 3'
- ordered by Austin (50μM)
Protein
- Coomassie (Bradford) Protein Assay Kit from Pierce
- Albumin standard from Pierce
- InVision™ His-tag In-gel Stain from Invitrogen (purchased in 2003?)
Dry ice
Dry ice is delivered on Tuesday mornings (before noon). The stash usually lasts all week but is gone some time over the weekend. There's not any remaining by Monday morning.
Stocks
Quartz cuvettes
For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes. I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the CD spectrometer at the Bioinstrumentation facility. The two microcell cuvettes were not suitable for CD ... they have high birefringence. The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm. However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm. So these cuvettes are not great for this purpose.
