Knight:Materials
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Knight Lab
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Common primers
VF2
5' tgc cac ctg acg tct aag aa 3'
VR
5' ATT ACC GCC TTT GAG TGA GC 3'
BioBricks-f
5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
- ordered by Reshma 5/1/2006 (25μM)
BioBricks-r
5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
- ordered by Reshma 8/24/2006
M13-R
5' cag gaa aca gct atg ac 3'
- ordered by Austin (50μM)
M13-F (-20)
5' GTA AAA CGA CGG CCA G 3'
- ordered by Austin (50μM)
Protein
- Coomassie (Bradford) Protein Assay Kit from Pierce
- Albumin standard from Pierce
- InVision™ His-tag In-gel Stain from Invitrogen (purchased in 2003?)
Yeast
Dry ice
Dry ice is delivered on Tuesday mornings (before noon). The stash usually lasts all week but is gone some time over the weekend. There's not any remaining by Monday morning.
