Knight:Orders: Difference between revisions
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* 8 well strip tubes, clear, low profile (but we don't need caps!) | * 8 well strip tubes, clear, low profile (but we don't need caps!) | ||
* Sigma M2128 Thiazolyl Blue Tetrazolium 500 mg | * Sigma M2128 Thiazolyl Blue Tetrazolium 500 mg | ||
* [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/K3007 K3007 N-(β-Ketocaproyl)-L-homoserine lactone] from Sigma | * [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/K3007 K3007 N-(β-Ketocaproyl)-L-homoserine lactone] from Sigma | ||
* Geneclean III kit, 600 preps, [http://www.mpbio.com/product_info.php?cPath=491_8_54_131_316&products_id=1001-600] | * Geneclean III kit, 600 preps, [http://www.mpbio.com/product_info.php?cPath=491_8_54_131_316&products_id=1001-600] | ||
* Mermaid kit, 200 preps, [http://www.mpbio.com/product_info.php?cPath=491_8_54_131_318&products_id=1005-200] | * Mermaid kit, 200 preps, [http://www.mpbio.com/product_info.php?cPath=491_8_54_131_318&products_id=1005-200] | ||
* large latex gloves | * large latex gloves | ||
==Placed Orders== | ==Placed Orders== | ||
* (3) NEB T4 DNA Ligase M0202S | |||
* NEB phi29 DNA polymerase M0269S | |||
*Req ID: 10855012 Ampicillin salt (Sigma A9518) | *Req ID: 10855012 Ampicillin salt (Sigma A9518) | ||
*Req ID: 10854533 [http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_intro TempliPhi 100] (Amersham/GE Healthcare) | *Req ID: 10854533 [http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_intro TempliPhi 100] (Amersham/GE Healthcare) |
Revision as of 06:39, 10 May 2007
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Needs to be Ordered
- 95% ethanol
- 8 well strip tubes, clear, low profile (but we don't need caps!)
- Sigma M2128 Thiazolyl Blue Tetrazolium 500 mg
- K3007 N-(β-Ketocaproyl)-L-homoserine lactone from Sigma
- Geneclean III kit, 600 preps, [1]
- Mermaid kit, 200 preps, [2]
- large latex gloves
Placed Orders
- (3) NEB T4 DNA Ligase M0202S
- NEB phi29 DNA polymerase M0269S
- Req ID: 10855012 Ampicillin salt (Sigma A9518)
- Req ID: 10854533 TempliPhi 100 (Amersham/GE Healthcare)
- 3 boxes Invitrogen PCR Supermix High Fidelity cat. 10790-020
- Req ID: 10854540
- two dozen black ultra-fine point Sharpies
- Req ID: 10854530
- case BD 305491 5 gallon sharps containers
- case Nalgene 5055-5015 microcentrifuge box, 64 places, foam insert
- 2 cs 10ul tips
- 6 cs 20ul tips
- 3 cs 200ul tips
- 3 cs 1000ul tips
- scotch tape. The rolls that can be put in existing dispensers.
Received Orders
(Please make sure to update this or tell Meagan if you receive an order -- and make sure to save the packing slip)
- ReqID 10851873
- Sigma D157805-5G N,N'-dimethylethylenediamine
- Invitrogen Sybr Safe
- PO# 10847064
- Nonidet-P40 substitute Sigma 74385-1L
- Tween-20 Sigma P7949-100ML
- Tween-40 Sigma P1504-100ML
- Tween-80 Sigma P8074-100ML
- Glycerol (Sigma G6279) -- on backorder
- 1.7mL clear sterile tubes (Reshma) --Meaganl 09:43, 12 April 2007 (EDT)
- Reshma 09:41, 19 March 2007 (EDT): Cartridges for Ptouch labeller. 18mm, 3/4", Black ink on white, TZ-241 TZ tape
- Trevigen TDG 4070-500-EB
- Trevigen MutY 4000-500-K
- Beckman Futura pH electrode, 511083 Calomel, 5mm x 225 mm VWR BK511083
- Beckman reference solution 4M KCl, 4 x 100 ml, 566468 VWR BK566068
- --Meaganl 14:44, 3 April 2007 (EDT)
- Reshma 11:55, 23 March 2007 (EDT): SpeI
- NEB EarI (yes again) R0528S
- NEB endo IV M0304S
- NEB endo VIII M0299S
- Qiagen miniprep kit (large)--Meaganl 10:32, 3 April 2007 (EDT)
- Immobilized Streptavidin Pierce #20349 (VWR # PI20349) --Meaganl 13:42, 26 March 2007 (EDT)
- Reshma 11:23, 19 March 2007 (EDT): Nalgene cryovials
- Reshma 17:06, 8 March 2007 (EST): 14 mL culture tubes Falcon 352059
- Reshma 13:44, 8 March 2007 (EST): Difco LB Lennox Broth
- NEB BsmBI R0580S --Meaganl 10:41, 22 March 2007 (EDT)
- Ambion superase-in #2694 --Meaganl 15:16, 15 March 2007 (EDT)
- Reshma: Qiagen ERC MinElute cleanup kit large #28206
- Reshma: PCR supermix
- --Meaganl 11:05, 9 March 2007 (EST)
- case BD sharps collector 3.1 liter 305488
- case standard petri dishes 100 x 15 mm VWR # 25384-250
- 3x bottles bleach
- 4 cases 1000 ul barrier pipet tips VWR 14217-730
- 4 cases 200 ul barrier pipet tips VWR 14217-728
- 3 cases 50 ml serological pipets BD 357550
- 3 cases 10 ml serological pipets BD 357551
- 1 case 25 ml serological pipets BD 357525
- NEB phi29 DNA polymerase M0269S --Meaganl 16:29, 8 March 2007 (EST)
- NEB BbsI R0539S --Meaganl 16:29, 8 March 2007 (EST)
- NEB EarI R0528S --Meaganl 16:29, 8 March 2007 (EST)
- Invitrogen Sybr Safe --Meaganl 16:27, 6 March 2007 (EST)
- NEB T4 DNA ligase large (M0202L) PO# 0010830023 --Meaganl 08:49, 2 March 2007 (EST)
- VWR 3 1/2 mm glass beads 1lb pack 26396-521 (PO# 10826298) tk 15:32, 26 February 2007 (EST)
- Invitrogen E-Gels, 0.8% EtBr, Single comb, pack 18, G5018-08 tk 15:32, 26 February 2007 (EST)
- PO #10825054
- PO# 10823861
- GE Healthcare Sephacryl S-500 HR 150 ml, Cat 17-0613-01 VWR 95016-920 tk 15:32, 26 February 2007 (EST)
- GE Healthcare Sephacryl S-400 HR 150 ml, Cat 17-0609-01 VWR 95016-912 tk 15:32, 26 February 2007 (EST)
- GE Healthcare Sephacryl S-300 HR 150 ml, Cat 17-0599-01 VWR 95016-908 tk 15:32, 26 February 2007 (EST)
- Polybead polystyrene red dyed microspheres, 0.2 micron, 2.5% aqueous solution, smallest amount tk 15:32, 26 February 2007 (EST)
- Order # 47T9282PPGGQ8LDHCRF9WDNN82
- PO#10822102
- Sucrose, Omnipure, EMD, VWR #EM-8510, 500g
- PO#10822065
- 3x pack 300 disposable spatula, standard, opaque, VWR 80081-190
- NEB BsmBI
- 2x NEB 2-log ladder, large N3200L
- P-10 tips (2 cases) --Meaganl 09:44, 2 February 2007 (EST)
- Multichannel 1200ul pipette tips --Meaganl 09:44, 2 February 2007 (EST)
- Bio-rad PCR strip tubes #TLS0801 (5 boxes) tk 19:13, 31 January 2007 (EST)
- NEB β Agarase I M0392S --Meaganl 14:37, 31 January 2007 (EST)
- Epicentre CircLigase #CL4111K --Meaganl 13:36, 30 January 2007 (EST)
- Micropure EZ columns Catalogue Number: 42530 from Millipore (shipped on 1/26)--Meaganl 13:36, 30 January 2007 (EST)
- Shipped on 1.26 --Meaganl 14:22, 29 January 2007 (EST)
- Qiagen RNase-free Dnase set Cat #79254
- Qiagen RNeasy minelute cleanup Cat #74204
- Qiagen ERC MinElute cleanup kit #28204
- EDTA disodium salt for molecular biology 500g Sigma E5134 --Meaganl 09:48, 24 January 2007 (EST)
- Sigma S7547-1Kg D-Sorbitol Sigmaultra --Meaganl 09:48, 24 January 2007 (EST)
- Sigma M6880-25G (malachite green oxalate salt) --Meaganl 09:48, 24 January 2007 (EST)
- DEPC - 25mL (Sigma) --Meaganl 09:48, 24 January 2007 (EST)
- 2x packs 100, BD Syringe 3 ml Luer Lok, BD 309585 --Meaganl 09:48, 24 January 2007 (EST)
- NEB N0345S Yeast Chromosome PFG Marker --Meaganl 21:36, 23 January 2007 (EST)
- NEB N0340S Lambda ladder PFG Marker --Meaganl 21:36, 23 January 2007 (EST)
- PMSF 100 mM in ethanol 50 ml Biochemika 93482 --Meaganl 14:56, 22 January 2007 (EST)
- N-lauroyl sarcosine sodium salt 30% solution 100 ml Biochemika 61747 --Meaganl 14:56, 22 January 2007 (EST)
- 2 cases standard petri dishes 100 x 15 mm VWR # 25384-250 --Meaganl 14:56, 22 January 2007 (EST)
- 4L cubetainer of 10x TBE buffer VWR EM-8820 --Meaganl 14:56, 22 January 2007 (EST)
- ClaI NEB R0197S --Meaganl 14:56, 22 January 2007 (EST)
- P-10 tips --Meaganl 14:56, 22 January 2007 (EST)
- 2 4L cubetainers of 10X TAE buffer Invitrogen #15558-026 --Meaganl 11:48, 18 January 2007 (EST)
- SYBR gold (Invitrogen catalog number S-11494) --Meaganl 11:48, 18 January 2007 (EST)
- Invitrogen Sybr Safe --Meaganl 11:48, 18 January 2007 (EST)
- Nunc Deepwell 384-well plates
- EcoR1, small (Robot)
- SpeI, large (Robot) x2
- PstI, small (Robot)
- Eppendorf Robot Miniprep kits x2 --Reshma 15:17, 10 January 2007 (EST)
- XbaI --Reshma 15:17, 10 January 2007 (EST)
- Difco LB Agar, Lennox--Meaganl 10:39, 9 January 2007 (EST)
- illustra TempliPhi 100 Amplification Kit (Amersham/GE Healthcare) tk 22:01, 22 December 2006 (EST)
- 200 ul pipet tips (3 cases)--Meaganl 07:46, 21 December 2006 (EST)
- 20 ul pipet tips (2 cases)--Meaganl 07:46, 21 December 2006 (EST)
- 1000 ul pipet tips (3 cases)--Meaganl 07:46, 21 December 2006 (EST)
- Falcon 14ml tubes 352059--Meaganl 07:46, 21 December 2006 (EST)
- AG 501-X8(D) Resin, molecular biology grade, 100 g, catalog number 143-6425 from Bio-Rad--Meaganl 07:46, 21 December 2006 (EST)
- Sigma Molecular Bio Grade 200 proof ethanol--Meaganl 10:12, 20 December 2006 (EST)
- RNaseZap wipes (Ambion AM9786)--Meaganl 10:12, 20 December 2006 (EST)
- Alpha Innotech (replacement) EtBr filter EBR-500K
- Qiagen miniprep kit (2 of these I believe) --Reshma 13:52, 9 December 2006 (EST)
- Eppendorf 30ml robot reagent reservoirs --Meaganl 14:22, 3 December 2006 (EST)
- 100ml sterile reagent reservoirs--Meaganl 11:26, 1 December 2006 (EST)
- (VWR) Nalgene microcentrifuge tube boxes (case)--Meaganl 11:26, 1 December 2006 (EST)
- Plastic disposible cuvettes, OPS, 10x10x45 mm, 2 optical surfaces, VWR 58017-825, case 500--Meaganl 11:26, 1 December 2006 (EST)
- Plastic disposible cuvettes, OPS, 10x4x45 mm, 2 optical surfaces, VWR 58017-847, case 500--Meaganl 11:26, 1 December 2006 (EST)
- Difco Bacto Agar 0140-01--Meaganl 11:26, 1 December 2006 (EST)
- Sigma L5263-25KU, Lyticase from Arthrobacter luteus, partially purified--Meaganl 15:33, 30 November 2006 (EST)
- Sigma 73660-5G Biochemika 99%+ 2-nitrophenyl β-D-galactopyranoside (ONPG)--Meaganl 15:44, 29 November 2006 (EST)
- SpeI NEB R0133S--Meaganl 15:04, 28 November 2006 (EST)
- NotI NEB R0189S--Meaganl 15:04, 28 November 2006 (EST)
- β-Agarase I NEB M0392S--Meaganl 15:04, 28 November 2006 (EST)
- Sigma 43816-50ml DL Dithiothreitol solution 1M Biochemika ultra -- tk 14:18, 27 November 2006 (EST)
- Sigma S7547-1Kg D-Sorbitol Sigmaultra --Meaganl 11:30, 27 November 2006 (EST)
- Sigma D5545-1G DL Dithiothreitol Sigmaultra --Meaganl 11:30, 27 November 2006 (EST)
- Brother Labeler 18mm 3/4" black ink on white tape --Meaganl 10:06, 27 November 2006 (EST)
- Nalgene microcentrifuge tube boxes (only got 4 boxes) --Meaganl 11:22, 20 November 2006 (EST)
- BD 60ml syringes --Meaganl 11:22, 20 November 2006 (EST)