Knight:Preparing stabs

1. Fill airtight, autoclavable vials with rubber or teflon caps 2/3 full of stab agar
2. Inoculate with a single colony repeatedly poking the inoculating loop into the agar
3. Incubate at 37°C for 8-12 hrs until cloudy tracks of bacteria become visible
4. Seal tightly and store in the dark at room temperature (15-22°C)
5. Revive by using inoculating loop to streak onto a new LB plate