Knight:Primers: Difference between revisions

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List any primers that might be generally useful here.
List any primers that might be generally useful here. These should be locatable in the "common primers" box.
 
===VF2===
 
5' tgc cac ctg acg tct aag aa 3'
 
===VR===
 
5' ATT ACC GCC TTT GAG TGA GC 3'
 
===VF2 (reverse)===
 
5' TTC TTA GAC GTC AGG TGG CA 3'
 
===VR (forward)===
 
5' GCT CAC TCA AAG GCG GTA AT 3'
 
===BioBricks-f===
 
5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
 
*ordered by [[Reshma Shetty | Reshma]] 5/1/2006 (25&mu;M)
 
===BioBricks-r===
 
5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
 
*ordered by [[Reshma Shetty | Reshma]] 8/24/2006
 
===Suffix-F===
 
5' A CTA GTA GCG GCC GCT GCA G 3'
 
*ordered by [[User:Meaganl|Meagan]]
 
===Prefix-R===
 
5' T CTA GAT GCG GCC GCG AAT TC 3'
 
*ordered by [[Meagan Lizarazo]]
 
===Suffix-F long===
 
5' GTT TCT TCA CTA GTA GCG GCC GCT GCA G 3'
 
*ordered by [[Reshma Shetty | Reshma]] 2/13/2007
 
===Prefix-R long===
 
5' GTT TCT TCT CTA GAT GCG GCC GCG AAT TC 3'
 
*ordered by [[Reshma Shetty | Reshma]] 2/13/2007
 
===M13-R===
 
5' cag gaa aca gct atg ac 3'
 
*ordered by [[Austin Che | Austin]] (50&mu;M)
 
===M13-F (-20)===
 
5' GTA AAA CGA CGG CCA G 3'
 
*ordered by [[Austin Che | Austin]] (50&mu;M)


* VF2: 5' tgc cac ctg acg tct aag aa 3'
* VR: 5' ATT ACC GCC TTT GAG TGA GC 3'
* VF2 (reverse): 5' TTC TTA GAC GTC AGG TGG CA 3'
** ordered by [[Reshma Shetty | Reshma]] 2/13/2007
* VR (forward): 5' GCT CAC TCA AAG GCG GTA AT 3'
** ordered by [[Reshma Shetty | Reshma]] 2/13/2007
* BioBricks-f: 5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
** ordered by [[Reshma Shetty | Reshma]] 5/1/2006 (25&mu;M)
* BioBricks-r: 5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
** ordered by [[Reshma Shetty | Reshma]] 8/24/2006
* Suffix-F: 5' A CTA GTA GCG GCC GCT GCA G 3'
** ordered by [[User:Meaganl|Meagan]]
* Prefix-R: 5' T CTA GAT GCG GCC GCG AAT TC 3'
** ordered by [[User:Meaganl|Meagan]]
* Suffix-F long: 5' GTT TCT TCA CTA GTA GCG GCC GCT GCA G 3'
** ordered by [[Reshma Shetty | Reshma]] 2/13/2007
* Prefix-R long: 5' GTT TCT TCT CTA GAT GCG GCC GCG AAT TC 3'
** ordered by [[Reshma Shetty | Reshma]] 2/13/2007
* M13-R: 5' cag gaa aca gct atg ac 3'
** ordered by [[Austin Che | Austin]] (50&mu;M)
* M13-F (-20): 5' GTA AAA CGA CGG CCA G 3'
** ordered by [[Austin Che | Austin]] (50&mu;M)
* T7 terminator: GCTAGTTATTGCTCAGCGG (50&mu;M) --[[User:Austin J. Che|Austin Che]] 13:55, 30 August 2007 (EDT)
* lacI-R: gtataacgttactggtttcac (50&mu;M) --[[User:Austin J. Che|Austin Che]] 13:55, 30 August 2007 (EDT)
* pET upstream: atgcgtccggcgtaga (50&mu;M) --[[User:Austin J. Che|Austin Che]] 13:55, 30 August 2007 (EDT)
* DuetUp2: ttgtacacggccgcataatc (50&mu;M) --[[User:Austin J. Che|Austin Che]] 13:55, 30 August 2007 (EDT)
* DuetDOWN1 GATTATGCGGCCGTGTACAA (50&mu;M) --[[User:Austin J. Che|Austin Che]] 13:55, 30 August 2007 (EDT)
* T7-VF2: GTTTCTGCtaatacgactcactatagggagaTGCCACCTGACGTCTAAGAA (50&mu;M) --[[User:Austin J. Che|Austin Che]] 10:39, 3 September 2007 (EDT)
* T7-VR: GTTTCTGCtaatacgactcactatagggagaATTACCGCCTTTGAGTGAGC (50&mu;M) --[[User:Austin J. Che|Austin Che]] 10:39, 3 September 2007 (EDT)
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Latest revision as of 07:39, 3 September 2007

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List any primers that might be generally useful here. These should be locatable in the "common primers" box.

  • VF2: 5' tgc cac ctg acg tct aag aa 3'
  • VR: 5' ATT ACC GCC TTT GAG TGA GC 3'
  • VF2 (reverse): 5' TTC TTA GAC GTC AGG TGG CA 3'
  • VR (forward): 5' GCT CAC TCA AAG GCG GTA AT 3'
  • BioBricks-f: 5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
  • BioBricks-r: 5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
  • Suffix-F: 5' A CTA GTA GCG GCC GCT GCA G 3'
  • Prefix-R: 5' T CTA GAT GCG GCC GCG AAT TC 3'
  • Suffix-F long: 5' GTT TCT TCA CTA GTA GCG GCC GCT GCA G 3'
  • Prefix-R long: 5' GTT TCT TCT CTA GAT GCG GCC GCG AAT TC 3'
  • M13-R: 5' cag gaa aca gct atg ac 3'
  • M13-F (-20): 5' GTA AAA CGA CGG CCA G 3'
  • T7 terminator: GCTAGTTATTGCTCAGCGG (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • lacI-R: gtataacgttactggtttcac (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • pET upstream: atgcgtccggcgtaga (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • DuetUp2: ttgtacacggccgcataatc (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • DuetDOWN1 GATTATGCGGCCGTGTACAA (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • T7-VF2: GTTTCTGCtaatacgactcactatagggagaTGCCACCTGACGTCTAAGAA (50μM) --Austin Che 10:39, 3 September 2007 (EDT)
  • T7-VR: GTTTCTGCtaatacgactcactatagggagaATTACCGCCTTTGAGTGAGC (50μM) --Austin Che 10:39, 3 September 2007 (EDT)
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