Knight:Protocols

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==DNA==
==DNA==
[[Knight:Agarose gel electrophoresis]]
[[Knight:Agarose gel electrophoresis]]
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[[Knight:Preparing electrocompetent cells]]
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Revision as of 22:03, 12 January 2006

CSAIL logo
Knight Lab



DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Restriction Digest

Knight:TOPO TA cloning

Knight:Site-directed mutagenesis

Escherichia coli

Knight:Preparing electrocompetent cells

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