Knight:Protocols

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[[Knight:Site-directed mutagenesis]]
[[Knight:Site-directed mutagenesis]]
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==Protein==
[[Knight:Purification of His-tagged proteins]] -- <font color=red>in progress!</font>
[[Knight:Purification of His-tagged proteins]] -- <font color=red>in progress!</font>
 +
 +
[[Knight:NuPAGE electrophoresis]] --<font color=red>in progress!</font>
==''Escherichia coli''==
==''Escherichia coli''==

Revision as of 11:52, 17 July 2006

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Knight Lab



Contents

DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Protein

Knight:Purification of His-tagged proteins -- in progress!

Knight:NuPAGE electrophoresis --in progress!

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Electroporation

Miscellaneous

Knight:Poster printing

Personal tools