Knight:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 49: Line 49:
==Miscellaneous==
==Miscellaneous==
 +
[[Knight:pH meter/Calibration|Calibrating the pH meter]]
 +
 +
[[Knight:pH meter/Measurement|Measuring pH]]
[[Knight:Poster printing]]
[[Knight:Poster printing]]
</div>
</div>

Revision as of 13:32, 18 August 2006

CSAIL logo
Knight Lab



Contents

DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Protein

Knight:Purification of His-tagged proteins -- in progress!

Knight:NuPAGE electrophoresis --in progress!

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Knight:Electroporation

Miscellaneous

Calibrating the pH meter

Measuring pH

Knight:Poster printing

Personal tools