Knight:Protocols

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==Protein==
==Protein==
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[[Knight:Purification of His-tagged proteins]]
+
[[Knight:Electrophoretic mobility shift assay]] --<font color=red>in progress!</font>
 +
 
 +
[[Knight:Microcon filtration of proteins]]
[[Knight:NuPAGE electrophoresis]]  
[[Knight:NuPAGE electrophoresis]]  
-
[[Knight:Electrophoretic mobility shift assay]] --<font color=red>in progress!</font>
+
[[Knight:Protein DNA binding]] --<font color=red>in progress!</font>
 +
 
 +
[[Knight:Purification of His-tagged proteins]]
==''Escherichia coli''==
==''Escherichia coli''==

Revision as of 09:19, 15 September 2006

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Knight Lab



Contents

DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Knight:Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Protein

Knight:Electrophoretic mobility shift assay --in progress!

Knight:Microcon filtration of proteins

Knight:NuPAGE electrophoresis

Knight:Protein DNA binding --in progress!

Knight:Purification of His-tagged proteins

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Knight:Electroporation

Miscellaneous

Calibrating the pH meter

Measuring pH

Knight:Poster printing

Personal tools