Knight:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Protein)
Line 36: Line 36:
==Protein==
==Protein==
[[Knight:Electrophoretic mobility shift assay]] --<font color=red>in progress!</font>
[[Knight:Electrophoretic mobility shift assay]] --<font color=red>in progress!</font>
 +
 +
[[Knight:Dialysis]]
[[Knight:Microcon filtration of proteins]]
[[Knight:Microcon filtration of proteins]]

Revision as of 17:19, 19 September 2006

CSAIL logo
Knight Lab



Contents

DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Knight:Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Protein

Knight:Electrophoretic mobility shift assay --in progress!

Knight:Dialysis

Knight:Microcon filtration of proteins

Knight:NuPAGE electrophoresis

Knight:Protein DNA binding --in progress!

Knight:Purification of His-tagged proteins

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Knight:Electroporation

Miscellaneous

Calibrating the pH meter

Measuring pH

Knight:Poster printing

Personal tools