Knight:Protocols

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==Protein==
==Protein==
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[[Knight:Protein solubility]] --<font color=red>in progress!</font>
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[[Knight:Centrifuge desalting]] --<font color=red>in progress!</font>
[[Knight:Centrifuge desalting]] --<font color=red>in progress!</font>

Revision as of 17:17, 28 September 2006

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Knight Lab



Contents

DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Knight:Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Protein

Knight:Protein solubility --in progress!

Knight:Centrifuge desalting --in progress!

Knight:Dialysis --in progress!

Knight:Electrophoretic mobility shift assay --in progress!

Knight:Gel filtration --in progress!

Knight:Microcon filtration of proteins --in progress!

Knight:NuPAGE electrophoresis

Knight:Protein DNA binding --in progress!

Knight:Purification of His-tagged proteins

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Knight:Electroporation

Miscellaneous

Calibrating the pH meter

Measuring pH

Knight:Poster printing

Personal tools