Knight:Protocols: Difference between revisions
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[[Knight:Preparing electrocompetent cells]] | [[Knight:Preparing electrocompetent cells]] | ||
==Miscellaneous== | |||
[[Knight:Poster printing]] | |||
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Revision as of 17:16, 12 May 2006
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DNA
Knight:Agarose gel electrophoresis
Annealing and primer extension
Ethanol precipitation of small DNA fragments
Knight:DNA ligation using NEB Quick Ligation Kit
Micropure EZ and Microcon purification
Phosphatase treatment of linearized vector
Knight:Addition of 3' A-overhangs
Knight:Site-directed mutagenesis
Escherichia coli
Knight:Preparing electrocompetent cells