Knight:Protocols: Difference between revisions
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[[Knight:Site-directed mutagenesis]] | [[Knight:Site-directed mutagenesis]] | ||
[[Knight:Purification of His-tagged proteins]] -- <font color=red>in progress!</font> | |||
==''Escherichia coli''== | ==''Escherichia coli''== | ||
Revision as of 10:48, 11 July 2006
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Knight Lab
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DNA
Knight:Agarose gel electrophoresis
Annealing and primer extension
Knight:Annealing and primer extension with Taq polymerase
Ethanol precipitation of small DNA fragments
Knight:DNA ligation using NEB Quick Ligation Kit
Micropure EZ and Microcon purification
Phosphatase treatment of linearized vector
Knight:Addition of 3' A-overhangs
Knight:Site-directed mutagenesis
Knight:Purification of His-tagged proteins -- in progress!
Escherichia coli
Preparing chemically competent cells (Inoue)
Transforming chemically competent cells (Inoue)
Knight:Preparing electrocompetent cells
