Knight:Protocols
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Knight Lab
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DNA
Knight:Agarose gel electrophoresis
Annealing and primer extension
Knight:Annealing and primer extension with Taq polymerase
Ethanol precipitation of small DNA fragments
Knight:DNA ligation using NEB Quick Ligation Kit
Knight:Micropure EZ and Microcon purification
Phosphatase treatment of linearized vector
Knight:Addition of 3' A-overhangs
Knight:Site-directed mutagenesis
Protein
Knight:Protein solubility --in progress!
Knight:Centrifuge desalting --in progress!
Knight:Dialysis --works poorly
Knight:Electrophoretic mobility shift assay --in progress!
Knight:Gel filtration --in progress!
Knight:Microcon filtration of proteins --works poorly
Knight:Protein DNA binding --in progress!
Knight:Purification of His-tagged proteins
Escherichia coli
Preparing chemically competent cells (Inoue)
Transforming chemically competent cells (Inoue)
Knight:Preparing electrocompetent cells
