Knight:Purification of His-tagged proteins
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| + | ==Overview== | ||
| + | "The high affinity of the Ni-NTA resins for 6xHis-tagged proteins or peptides is due to both the specificity of the interaction between histidine residues and immobilized nickel ions and to the strength with which these ions are held to the NTA resin." <cite>QiagenNTAManual</cite> | ||
| + | |||
| + | ==Protocols== | ||
*[[Knight:Purification of His-tagged proteins/Denaturing]] | *[[Knight:Purification of His-tagged proteins/Denaturing]] | ||
*[[Knight:Purification of His-tagged proteins/Native]] <font color=red>(in progress!)</font> | *[[Knight:Purification of His-tagged proteins/Native]] <font color=red>(in progress!)</font> | ||
| + | |||
| + | ==References== | ||
| + | <biblio> | ||
| + | #SauerDenaturingProtocol [[Sauer:Purification of His-tagged proteins]] | ||
| + | #QiagenNTAManual [http://www1.qiagen.com/literature/handbooks/PDF/Protein/Purification/NiNTA_Spin/1023679HBNINTA_022003WW.pdf Qiagen Ni NTA Spin Kit manual] | ||
| + | </biblio> | ||
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Revision as of 16:49, 24 September 2006
| Knight Lab
|
Overview
"The high affinity of the Ni-NTA resins for 6xHis-tagged proteins or peptides is due to both the specificity of the interaction between histidine residues and immobilized nickel ions and to the strength with which these ions are held to the NTA resin." [1]
Protocols
- Knight:Purification of His-tagged proteins/Denaturing
- Knight:Purification of His-tagged proteins/Native (in progress!)
