Knight:RNA work: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
#[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=111 RNaseZap] (Ambion) | #[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=111 RNaseZap] (Ambion) | ||
#[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=112 RNaseZap wipes] (Ambion) | #[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=112 RNaseZap wipes] (Ambion) | ||
#DEPC (should be stored at 4°C) | #[[DEPC]] (should be stored at 4°C) | ||
#*DMPC might be a better choice since it is essentially equivalent but may be less carcinogenic than DEPC | #*DMPC might be a better choice since it is essentially equivalent but may be less carcinogenic than DEPC | ||
#RNase free water | #RNase free water | ||
Revision as of 11:47, 8 December 2006
A list of various items that might be useful while working with RNA.
The sterile hood in 306 is dedicated to RNA work.
- RNaseZap (Ambion)
- RNaseZap wipes (Ambion)
- DEPC (should be stored at 4°C)
- DMPC might be a better choice since it is essentially equivalent but may be less carcinogenic than DEPC
- RNase free water
- The DI water may be sufficiently free of RNases
- 3% Hydrogen peroxide
- Oven for baking glassware
- Note that caps can be heated only to 140°C but removal of RNAases requires 180°C
- Decontaminated electrophoresis apparatus and power supply
- RNA ladder
- SYBR gold
- Sensitive nucleic acid stain.
- RNase free 10X TBE buffer
- For native gels
- Glyoxal and formamide
- For denaturing gels
