Knight:RNA work: Difference between revisions
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A list of various items that might be useful while working with RNA. | A list of various items that might be useful while working with RNA. | ||
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#[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=111 RNaseZap] (Ambion) | #[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=111 RNaseZap] (Ambion) | ||
#[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=112 RNaseZap wipes] (Ambion) | #[http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=112 RNaseZap wipes] (Ambion) | ||
#[[ | #[[DMPC]] (less carcinogenic than [[DEPC]]) | ||
#[http://www.ambion.com/catalog/CatNum.php?AM1964 RNaseAlert Kit] (Ambion) | |||
#RNase free water | #RNase free water | ||
#*The DI water may be sufficiently free of RNases | #*The DI water may be sufficiently free of RNases | ||
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#*Note that caps can be heated only to 140°C but removal of RNAases requires 180°C | #*Note that caps can be heated only to 140°C but removal of RNAases requires 180°C | ||
#Decontaminated electrophoresis apparatus and power supply | #Decontaminated electrophoresis apparatus and power supply | ||
#RNA ladder | #[http://www.neb.com/nebecomm/products/productN0362.asp RNA ladder] | ||
#[[SYBR | #[[SYBR Gold]] | ||
#*Sensitive nucleic acid stain. | #*Sensitive nucleic acid stain. | ||
#RNase free 10X TBE buffer | #RNase free [[10X TBE]] buffer | ||
#*For native gels | #*For native gels | ||
#Glyoxal and formamide | #Glyoxal and formamide | ||
#*For denaturing gels | #*For denaturing gels | ||
#RNase free tips and tubes | |||
#[http://www.neb.com/nebecomm/products/productM0303.asp RNase free DNase I] (We have [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D4513 Deoxyribonuclease I from bovine pancreas]. Is this good enough for RNA work? Stored at -20°C in the large freezer on the door shelf.) | |||
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Latest revision as of 13:45, 14 December 2006
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Knight Lab
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A list of various items that might be useful while working with RNA.
The sterile hood in 306 is dedicated to RNA work.
- RNaseZap (Ambion)
- RNaseZap wipes (Ambion)
- DMPC (less carcinogenic than DEPC)
- RNaseAlert Kit (Ambion)
- RNase free water
- The DI water may be sufficiently free of RNases
- 3% Hydrogen peroxide
- Oven for baking glassware
- Note that caps can be heated only to 140°C but removal of RNAases requires 180°C
- Decontaminated electrophoresis apparatus and power supply
- RNA ladder
- SYBR Gold
- Sensitive nucleic acid stain.
- RNase free 10X TBE buffer
- For native gels
- Glyoxal and formamide
- For denaturing gels
- RNase free tips and tubes
- RNase free DNase I (We have Deoxyribonuclease I from bovine pancreas. Is this good enough for RNA work? Stored at -20°C in the large freezer on the door shelf.)
