Knight:Reconstituting primers

From OpenWetWare

Revision as of 10:12, 7 May 2010 by Reshma P. Shetty (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search



Invitrogen recommends the following reconstitution procedure -

  1. Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
  2. To make a 25 μM stock, add YμL of sterile H2O to X nmoles of dry primer stock. (See equations below).
  3. Allow to sit for 2 mins, then vortex for 15 secs.

Short version

Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer

The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.

Long version

Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer


See Reconstituting primers for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.

Personal tools