Knight:TOPO TA cloning

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==Notes==
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*[[Tom Knight]] claims you can cut the reaction volume in half and it will still work fine.
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*Use fresh PCR product for best results.  (The A overhang tends to get chewed back over time).
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==Resources==
==Resources==
*[http://www.invitrogen.com/content.cfm?pageid=4073&CFID=2390357&CFTOKEN=58806917 Website]
*[http://www.invitrogen.com/content.cfm?pageid=4073&CFID=2390357&CFTOKEN=58806917 Website]

Revision as of 11:03, 29 September 2005

Notes

  • Tom Knight claims you can cut the reaction volume in half and it will still work fine.
  • Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).

Resources

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