Knight:TOPO TA cloning

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*[http://www.invitrogen.com/content/sfs/ProductNotes/F_TOPO%20RD-MKT-TL-HL050810.pdf?ProductNoteId=43 Product notes]
*[http://www.invitrogen.com/content/sfs/ProductNotes/F_TOPO%20RD-MKT-TL-HL050810.pdf?ProductNoteId=43 Product notes]
*[http://www.invitrogen.com/content/sfs/brochures/710_021849%20_B_TOPOCloning_bro.pdf Brochure]
*[http://www.invitrogen.com/content/sfs/brochures/710_021849%20_B_TOPOCloning_bro.pdf Brochure]
 +
*[http://www.invitrogen.com/search.cfm?category=Vectors&searchTerm=pCR4%2DTOPO&id=243 pCR4-TOPO]

Revision as of 12:36, 29 September 2005

Notes

  • Tom Knight claims you can cut the reaction volume in half and it will still work fine.
  • Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).

Resources

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