Knight:TOPO TA cloning

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Revision as of 17:30, 29 September 2005 by JeremyZucker (Talk | contribs)
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  • Tom Knight claims you can cut the reaction volume in half and it will still work fine.
  • Use fresh PCR product for best results. (The A overhang tends to get chewed back over time ... even using day old PCR product can reduce efficiency.)
  • Your primers should NOT have 5' phosphates. They interfere with topoisomerase I.


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