Knight:TOPO vector preparation

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Revision as of 19:35, 6 June 2007 by Reshma P. Shetty (talk | contribs) (New page: ==Overview== This is a made-up protocol to prepare pSB**5 vectors for TOPO cloning. It has not been verified at all! ==Materials== *[[Syntheti...)
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Overview

This is a made-up protocol to prepare pSB**5 vectors for TOPO cloning. It has not been verified at all!

Materials

Procedure

Nick plasmid

  1. Mix
    • X μL plasmid
    • 1 μL Nt.BstNB I
    • 5 μL NEBuffer 3
    • 44-X μL H2O
  2. Incubate at 55°C for several hours
  3. Heat-inactivate at 80°C for 20 mins
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