Knight:pRML1/2-sequencing: Difference between revisions
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===pRML1=== | ===pRML1=== | ||
New primers for pRML1 9/17/06 | |||
New primers 9/17/06 | |||
ACCTGACTACGGCATCTCTG,TKR1 (46160) | ACCTGACTACGGCATCTCTG,TKR1 (46160) | ||
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September 24 primers | September 24 primers for pRML1 | ||
GCTCAATGTTGACTAGCCAGC,CENF | GCTCAATGTTGACTAGCCAGC,CENF | ||
Line 145: | Line 66: | ||
===pRML2=== | ===pRML2=== | ||
Latest revision as of 17:28, 9 October 2006
Sequencing primers for pRML1, pRML2:
- T3 for pRML1
- T7 for pRML2
For both pRML1 & 2:
- attgtctcatgagcggatac,ampR-F
- ggtcacgctgcgcgtaacc,F1-F (works on pRML1, missing region on pRML2)
- atggaaaaacgccagcaacg,322ori-R
Construction primers:
- pRML1 (Left YAC arm)
- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGCTGGGCCATTCTCATGAAG,pRML1-R
- biobrick right end, binding site for Cen4 sequence
- binds with stutter
- pRML2-F (Right YAC arm)
- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ACGCGCGTCGACGGTATCGG,pRML2-F
- biobrick left end, ARSH4 binding site
- binds with stutter
pRML1
New primers for pRML1 9/17/06
ACCTGACTACGGCATCTCTG,TKR1 (46160)
CACGAACCATAAACCATTCC,TK1 (46360)
ttgcctgggctttggacgtc,TKR2 (46860)
cagataccgcaccgtattgg,TK2 (47060)
caccacacaacaccgcctcg,TKR3 (47570)
tggtcgaacgcagacgcgtg,TK3 (47760)
cacaggtagttctggtccattg,TRP1 (460) pSH63 AF298789
agcatcggaatctagagcac,TRPR1 (550)
ggtgttgatgtaagcggagg,TRP2 (1060)
ctatttcttagcatttttgacg,TRPR2 (1130)
acatcaacaccaataacgcc,YAC4R1 (9710) pYAC4
gcttacattt tatgttagct gg,YAC4-1 (9640)
agcgttgcct catcaatgcg,YAC4-2 (10370)
cacacagtggaccgaacgtg,YAC4R2
GGAAATTCATCGATTTCGAAG,T3R
September 24 primers for pRML1
GCTCAATGTTGACTAGCCAGC,CENF
TGATCGCGTAGTCGATAGTGG,TRPR0
GCGGCACGCTGTTGACGCTG,TK4
pRML2
Primers 9/17/06
aaatt gaagctctaa tttgtgag,URA1 (3200) DQ882849
ggtagagggtgaacgttacag,URA1R (3300)
GGAAATTCATCGATTTCGAAGC,T7R
September 24 primers:
GCAGGCATGCCTGCAGGTCGAG,oriR2
TACCTGAGAGCAGGAAGAGC,ARS
Final sequence, pRML1=
>pRML1 9742 bp ggatccatatgactagtgatcctctagagtcgatcgacctgcaggcatgcctgcaggtcg aggcgataagcttcatttttagataaaatttattaatcatcattaatttcttgaaaaaca ttttatttattgatcttttataacaaaaaacccttctaaaagtttatttttgaatgaaaa acttataaaaatttatgaaaactacaaaaaataaaatttttaattaaaataattttgata agaacttcaatctttgactagcttagtcatttttgagatttaattaatattttatgttta ttcatatataaactattcaaaatattatagaatttaaacattttaacatcttaatcattc ataaataactaaaaatcaaagtattacattcaataaataacttttactcaatgtcaaaga aattattggggttggggttggggttggggttggggttggggttggggttggggttggggt tggggttggggttggggttggggttggggttggggttggggttggggttggggttggggt tggggttggggttggggttggggttggggttggggttggggttggggttggggttggggt tggggttggggttggggttgggggttggggttggggttggggttggggttggggttgggg ttggggttggggttggggttggggttggggtctgcctcgcgcggaaattcatcgatttcg aagctagctcgcgagtcgaccgcggccgcagatctgaattccctttagtgagggttaatt tacgtagcgatgataagctgtcaaacatgagctgggccattctcatgaagaatatcttga atttattgtcatattactagctagttggtgtggaagtcctaatatcggtgatcaatatag tggttgacatgctggctagtcaacattgagccttttgatcatgcaaatatattacggtat tttacaatcaaatatcaaacttaactattgactttataacttatttaggtggtaacattc ttataaaaaagaaaaaaattactgcaaaacagtactagcttttaacttgtatcctaggtt atctatgctgtctcaccatagagaatattacctatttcagaatgtatgtccatgattcgc cgggtaaatacatataatacacaaatctggcttaataaagtctataatatatctcataaa gaagtgctaaattggctagtgctatatatttttaagaaaatttcttttgactaagtccat atcgactttgtaaaagttcacattagcatacatatattacacgagccagaaatagtaact tttgcctaaatcacaaattgcaaaawttaattgcttgcaaaaggtcacatgcttataatc aacttttttaaaaatttaaaatacttttttattttttatttttaaacataaatgaaataa tttatttattgtttatgattaccgaaacataaaacctgctcaagaaaaagaaactgtttt gtccttggaaaaaaagcactacctaggagcggccaaaatgccgaggctttcatagcttaa actctttacagaaaataggcattatagatcagttcgagttttcttattcttccttccggt tttatcgtcacagttttacagtaaataagtatcacctcttagagttcgacccggggtttt ttctccttgacgttaaagtatagaggtatattaacaattttttgttgatacttttattac atttgaataagaagtaatacaaaccgaaaatgttgaaagtattagttaaagtggttatgc agtttttgcatttatatatctgttaatagatcaaaaatcatcgcttcgctgattaattac cccagaaataaggctaaaaaactaatcgcattatcatcctatggttgttaatttgattcg ttcatttgaaggtttgtggggccaggttactgccaatttttcctcttcataaccataaaa gctagtattgtagaatctttattgttcggagcagtgcggcgcgaggcacatctgcgtttc aggaacgcgaccggtgaagacgaggacgcacggaggagagtcttccttcggagggctgtc acccgctcggcggcttctaatccgtactatgagataagcaagtatcatctcatttcatta cctgaagtcgagtaaacagaaaatccaattgttgatgaacctcaatgacttagaactatc tatcggcagatcatataaagaggatttagaattaattccacatgttaaaatagtgaagga gcatgttcggcacacagtggaccgaacgtggggtaagtgcactagggtccggttaaacgg atctcgcattgatgaggcaacgctaattatcaacatatagattgttatctatctgcatga acacgaaatctttacttgacgacttgaggctgatggtgtttatgcaaagaaaccactgtg tttaatatgtgtcactgtttgatattactgtcagcgtagaagataatagtaaaagcggtt aataagtgtatttgagataagtgtgataaagtttttacagcgaaaagacgataaatacaa gaaaatgattacgaggatacggagagaggtatgtacatgtgtatttatatactaagctgc cggcggttgtttgcaagaccgagaaaaggctagcaagaatcgggtcattgtagcgtatgc gcctgtgaacattctcttcaacaagtttgattccattgcggtgaaatggtaaaagtcaac cccctgcgatgtatattttcctgtacaatcaatcaaaaagccaaatgatttagcattatc tttacatcttgttattttacagattttatgtttagatcttttatgcttgcttttcaaaag gcctgcaggcaagtgcacaaacaatacttaaataaatactactcagtaataacctatttc ttagcatttttgacgaaatttgctattttgttagagtcttttacaccatttgtctccaca cctccgcttacatcaacaccaataacgccatttaatctaagcgcatcaccaacattttct ggcgtcagtccaccagctaacataaaatgtaagctttcggggctctcttgccttccaacc cagtcagaaatcgagttccaatccaaaagttcacctgtcccacctgcttctgaatcaaac aagggaataaacgaatgaggtttctgtgaagctgcactgagtagtatgttgcagtctttt ggaaatacgagtcttttaataactggcaaaccgaggaactcttggtattcttgccacgac tcatctccatgcagttggacgatatcaatgccgtaatcattgaccagagccaaaacatcc tccttaggttgattacgaaacacgccaaccaagtatttcggagtgcctgaactattttta tatgcttttacaagacttgaaattttccttgcaataaccgggtcaattgttctctttcta ttgggcacacatataatacccagcaagtcagcatcggaatctagagcacattctgcggcc tctgtgctctgcaagccgcaaactttcaccaatggaccagaactacctgtgaaattaata acagacatactccaagctgcctttgtgtgcttaatcacgtatactcacgtgctcaatagt caccaatgccctccctcttggccctctccttttcttttttcgaccgaattaattcttaat cggcaaaaaaagaaaagctccggatcaagattgtacgtaaggtgacaagctatttttcaa taaagaatatcttccactactgccatctggcgtcataactgcaaagtacacatatattac gatgctgttctattaaatgcttcctatattatatatatagtaatgtcgttaaccgttgat ttttactgattggtctctgccgtgcgggtgagcggcgcgtccagacgggcgggtgcacgt ttctttttctgaccatttccctgtcagctcttttagatcggggctgccgtttgaaaagtt ttatcatcgccagacatgccataaggaatatctccaccgcctctaactgtccctaactcg gtctctttcttataactaccattactcagtgttgagagattggtagaaatatacgcgtat acgttcattcaagacttattttcagccagtaatattccctactcctcttccgttttcaga atagagatggaccagccaaggacccattcaggacccacgacggccagcaatcctgccccc agctccaccaactcgtcatctgctcccagcgcgactaattcaaagcaggaaagatccaca ggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagca ggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaatt gcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatgga cgataattcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcat gataataatggtttcttagaatttaattcgaacacgcagatgcagtcggggcggcgcggt cccaggtccacttcgcatattaaggtgacgcgtgtggcctcgaataccgagcgaccctgc agcgacccgcttaacagcgtcaacagcgtgccgcagatctctcttgcgagatgatcccgc attttcttgaaagctttgcagaggctagcagaattaccctccacgttgattgtctgcgag gcaagaatgatcatcaccgtagtgagagtgcgttcaaggctcttgcggttgccataagag aagccacctcgcccaatggtaccaacgatgttccctccaccaaaggtgttcttatgtagt gacaccgattatttaaagctgcagcatacgatatatatacatgtgtatatatgtatacct atgaatgtcagtaagtatgtatacgaacagtatgatactgaagatgacaaggtaatgcat cattctatacgtgtcattctgaacgaggcgcgctttccttttttctttttgctttttctt tttttttctcttgaactcgagaaaaaaaatataaaagagatggaggaacgggaaaaagtt agttgtggtgataggtggcaagtggtattccgtaagaacaacaagaaaagcatttcatat tatggctgaactgagcgaacaagtgcaaaatttaagcatcaacgacaacaacgagaatgg ttatgttcctcctcacttaagaggaaaaccaagaagtgccagaaataacagtagcaacta caataacaacaacggcggctacaacggtggccgtggcggtggcagcttctttagcaacaa ccgtcgtggtggttacggcaacggtggtttcttcggtggaaacaacggtggcagcagatc ttggtggcgtgaaactcccgcacctcttcggccagcgccttgtagaagcgcgtatggctt cgtacccctgccatcaacacgcgtctgcgttcgaccaggctgcgcgttctcgcggccata acaaccgacgtacggcgttgcgccctcgccggcaacaaaaagccacggaagtccgcctgg agcagaaaatgcccacgctactgcgggtttatatagacggtccccacgggatggggaaaa ccaccaccacgcaactgctggtggccctgggttcgcgcgacgatatcgtctacgtacccg agccgatgacttactggcgggtgttgggggcttccgagacaatcgcgaacatctacacca cacaacaccgcctcgaccagggtgagatatcggccggggacgcggcggtggtaatgacaa gcgcccagataacaatgggcatgccttatgccgtgaccgacgccgttctggctcctcata tcgggggggaggctgggagctcacatgccccgcccccggccctcaccctcatcttcgacc gccatcccatcgccgccctcctgtgctacccggccgcgcgataccttatgggcagcatga ccccccaggccgtgctggcgttcgtggccctcatcccgccgaccttgcccggcacaaaca tcgtgttgggggcccttccggaggacagacacatcgaccgcctggccaaacgccagcgcc ccggcgagcggcttgacctggctatgctggccgcgattcgccgcgtttatgggctgcttg ccaatacggtgcggtatctgcagggcggcgggtcgtggcgggaggattggggacagcttt cgggggcggccgtgccgccccagggtgccgagccccagagcaacgcgggcccacgacccc atatcggggacacgttatttaccctgtttcgggcccccgagttgctggcccccaacggcg acctgtataacgtgtttgcctgggctttggacgtcttggccaaacgcctccgtcccatgc acgtctttatcctggattacgaccaatcgcccgccggctgccgggacgccctgctgcaac ttacctccgggatggtccagacccacgtcaccaccccaggctccataccgacgatctgcg acctggcgcgcacgtttgcccgggagatgggggaggctaactgaaacacggaaggagaca ataccggaaggaacccgcgctatgacggcaataaaaagacagaataaaacgcacgggtgt tgggtcgtttgttcataaacgcggggttcggtcccagggctggcactctgtcgatacccc accgagaccccattgggaccaatacgcccgcgtttcttccttttccccaccccaaccccc aagttcgggtgaaggcccagggctcgcagccaacgtcggggcggcaagccctgccatagc cacgggccccgtgggttagggacggggtcccccatggggaatggtttatggttcgtgggg gttattattttgggcgttgcgtggggtcaggtccacgactggactgagcagacagaccca tggtttttggatggcctgggcatggaccgcatgtactggcgcgacacgaacaccgggcgt ctgtggctgccaaacacccccgacccccaaaaaccaccgcgcggatttctggcgccgccg gacgaactaaacctgactacggcatctctgccccttcttcgctggtacgaggagcgcttt tgttttgtattggtcaccacggccgagtttccgcgggaccccggccagctgctttacatc ccgaagacctacctgctcggccggcccccgaacgcgagcctgcccgcccccaccacggtc gagccgaccgcccagcctcccccctcggtcgccccccttaagggtctcttgcacaatcca gccgcctccgtgttgctgcgttcccgggcctgggtaacgttttcggccgtccctgacccc gaggccctgacgttcccgcggggagacaacgtggcgacggcgagccacccgagcgggccg cgtgatacaccgcccccccgaccgccggttggggcccggcggcacccgacgacggagctg gacatcacgcacctgcacaacgcgtccacgacctggttggccacccggggcctgttgaga tccccaggtaggtacgtgtatttctccccgtcggcctcgacgtggcccgtgggcatctgg acgacgggggagctggtgctcgggtgcgatgccgcgctggtgcgcgcgcgctacgggcgg gaattaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaa atacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatat tgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg gcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaa gatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatcctt gagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgt ggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactat tctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatg acagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaactta cttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggat catgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgag cgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaa ctacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgca ggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagcc ggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgt atcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatc gctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatat atactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctt tttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagac cccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgc ttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctacca actctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttcta gtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgct ctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttg gactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgc acacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcta tgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagg gtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagt cctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggggg cggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctgg ccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattacc gcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtg agcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatt tcacaccgcatacggtacccgg
Final sequence, pRML2
>pRML2 5180 bp ggatcctctagagtcgatcgacctgcaggcatgcctgcaggtcgaggcgataagcttcat ttttagataaaatttattaatcatcattaatttcttgaaaaacattttatttattgatct tttataacaaaaaacccttctaaaagtttatttttgaatgaaaaacttataaaaatttat gaaaactacaaaaaataaaatttttaattaaaataattttgataagaacttcaatctttg actagcttagtcatttttgagatttaattaatattttatgtttattcatatataaactat tcaaaatattatagaatttaaacattttaacatcttaatcattcataaataactaaaaat caaagtattacattcaataaataacttttactcaatgtcaaagaaattattggggttggg gttggggttggggttggggttggggttggggttggggttggggttggggttggggttggg gttggggttggggttggggttggggttggggttggggttggggttggggttggggttggg gttggggttggggttggggttggggttggggttggggttggggttggggttggggttggg gttggggttgggggttggggttggggttggggttggggttggggttggggttggggttgg ggttggggttggggttggggtctgcctcgcgcggaaattcatcgatttcgaagctagctc gcgagtcgaccgccggcgcagatctgaattcgatgtacccaattcgccctatagtgagtc gtattacgcgcgtcgacggtatcgggggatcgccaacaaatactaccttttatcttgctc ttcctgctctcaggtattaatgccgaattgtttcatcttgtctgtgtagaagaccacaca cgaaaatcctgtgattttacattttacttatcgttaatcgaatgtatatctatttaatct gcttttcttgtctaataaatatatatgtaaagtacgctttttgttgaaattttttaaacc tttgtttatttttttttcttcattccgtaactcttctaccttctttatttactttctaaa atccaaatacaaaacataaaaataaataaacacagagtaaattcccaaattattccatca ttaaaagatacgaggcgcgtgtaagttacaggcaagcgatccgtcgataagctttttctt tccaattttttttttttcgtcattataaaaatcattacgaccgagattcccgggtaataa ctgatataattaaattgaagctctaatttgtgagtttagtatacatgcatttacttataa tacagttttttagttttgctggccgcatcttctcaaatatgcttcccagcctgcttttct gtaacgttcaccctctaccttagcatcccttccctttgcaaatagtcctcttccaacaat aataatgtcagatcctgtagagaccacatcatccacggttctatactgttgacccaatgc gtctcccttgtcatctaaacccacaccgggtgtcataatcaaccaatcgtaaccttcatc tcttccacccatgtctctttgagcaataaagccgataacaaaatctttgtcgctcttcgc aatgtcaacagtacccttagtatattctccagtagatagggagcccttgcatgacaattc tgctaacatcaaaaggcctctaggttcctttgttacttcttctgccgcctgcttcaaacc gctaacaatacctgggcccaccacaccgtgtgcattcgtaatgtctgcccattctgctat tctgtatacacccgcagagtactgcaatttgactgtattaccaatgtcagcaaattttct gtcttcgaagagtaaaaaattgtacttggcggataatgcctttagcggcttaactgtgcc ctccatggaaaaatcagtcaagatatccacatgtgtttttagtaaacaaattttgggacc taatgcttcaactaactccagtaattccttggtggtacgaacatccaatgaagcacacaa gtttgtttgcttttcgtgcatgatattaaatagcttggcagcaacaggactaggatgagt agcagcacgttccttatatgtagctttcgacatgatttatcttcgtttcctgcaggtttt tgttctgtgcagttgggttaagaatactgggcaatttcatgtttcttcaacactacatat gcgtatatataccaatctaagtctgtgctccttccttcgttcttccttctgttcggagat taccgaatcaaaaaaatttcaaggaaaccgaaatcaaaaaaaagaataaaaaaaaaatga tgaattgaaaagcttgatatcgaattaattcgcgctcactggccgtcgttttacaacgtc gtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcg ccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcc tgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggtta cgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcc cttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctt tagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatg gttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtcca cgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtct attcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctga tttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcac ttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatat gtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagag tatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcc tgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgc acgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccc cgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatc ccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactt ggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaatt atgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgat cggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgcct tgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgat gcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagc ttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcg ctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtc tcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatcta cacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgc ctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattga tttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcat gaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagat caaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaa accaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaa ggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagtt aggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgtt accagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgata gttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagctt ggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccac gcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggaga gcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcg ccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaa aaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat gttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagc tgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcgga agagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagtta gctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgg aattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcg cgggccgctctagaactagt