Koch HTS Facility:Instrumentation: Difference between revisions
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[[image:Incubator.jpg|thumb|Incubator]] | [[image:Incubator.jpg|thumb|Incubator]] | ||
*Automated cell plating, administration and removal of compounds via integrated automated CO2, temp, and humidity incubator | *Automated cell plating, administration and removal of compounds via integrated automated CO2, temp, and humidity incubator | ||
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===Integrated Robotic Automated Assay Platform=== | ===Integrated Robotic Automated Assay Platform=== | ||
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*Automated microplate centrifuge | *Automated microplate centrifuge | ||
*Integrated with Tecan M1000 Plate Reader, Arrayscan Screening Microscope (see below for details) | *Integrated with Tecan M1000 Plate Reader, Arrayscan Screening Microscope (see below for details) | ||
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===Tecan M1000 Plate Reader=== | ===Tecan M1000 Plate Reader=== | ||
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*AlphaScreen and AlphaLISA technology | *AlphaScreen and AlphaLISA technology | ||
*NanoQuant Plate compatibility for nucleic acid quantification | *NanoQuant Plate compatibility for nucleic acid quantification | ||
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===Arrayscan Screening Microscope | ===Arrayscan VTI High Content Screening Microscope=== | ||
*LED Light Engine designed for high performance excite blue (e.g. Hoechst), green (e.g. FITC, GFP), orange red (e.g. TRITC), deep red (e.g.. Texas Red), and long red (e.g. Cy5) fluorochromes | *LED Light Engine designed for high performance excite blue (e.g. Hoechst), green (e.g. FITC, GFP), orange red (e.g. TRITC), deep red (e.g.. Texas Red), and long red (e.g. Cy5) fluorochromes | ||
* | *5X-10X-20X objectives | ||
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===Bl2+ Lentiviral shRNA Screening Platform=== | ===Bl2+ Lentiviral shRNA Screening Platform=== | ||
*BL2+ cell culture hoods and 4 HeraCell incubators | *BL2+ cell culture hoods and 4 HeraCell incubators | ||
*Tecan Evo 150 liquid handling deck for cell plating and lentiviral manipulation | *Tecan Evo 150 liquid handling deck for cell plating and lentiviral manipulation | ||
*Biotek EL406 Plate Washer | |||
*KBio WASP Plate Sealer | |||
*Agilent VSpin Centrifuge with Access2 robotic loader | |||
*Enclosed robotics platform for packaging, manipulation and screening of lentiviral shRNA (RNAi) vectors integrated with a Liconic incubator for storage after cell plating | *Enclosed robotics platform for packaging, manipulation and screening of lentiviral shRNA (RNAi) vectors integrated with a Liconic incubator for storage after cell plating | ||
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===Tecan Evo 100 Liquid Handler=== | ===Tecan Evo 100 Liquid Handler=== | ||
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====High-Throughput Flow Cytometer==== | ====High-Throughput Flow Cytometer==== | ||
* | * high throughput flow cytometer that uses a patented "air gap delimited" aspiration using a contact sensing probe to map microplane<br> | ||
* two solid state lasers: blue (488 nm) and red (633 nm); four simultaneous collection channels; FL1 - 533/30 nm, FL2 - 585/40 nm, FL3 - > 670 nm, FL4 - 675/25 nm<br> | |||
* compatible with 96 or 384-well formats<br> | |||
* small sampling volumes, as low at 2 ul; can read a 96-well plate in as little as 5 minutes and a 384-well plate in 25 minutes<br> | |||
* used for small molecule screening, live-dead assays and antibody binding experiments<br> | |||
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Latest revision as of 11:09, 13 July 2017
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Koch HTS Facility InstrumentationTwo Evo 150 Liquid Handling Decks In Baker Hood (HEPA) Enclosures
Integrated Robotic Automated Assay Platform
Tecan M1000 Plate Reader
Arrayscan VTI High Content Screening Microscope
Bl2+ Lentiviral shRNA Screening Platform
Tecan Evo 100 Liquid Handler
Tecan Evo 100 Liquid Handler
Tecan Evo 150 Liquid Handler
BioTek El406 Plate Washer/Dispenser
Agilent VSpin with Access2
Thermo ArraySCAN VTI
Thermo Infinite M1000 Plate Reader
Intellicyt iQue ScreenerHigh-Throughput Flow Cytometer
BL2+ EquipmentTecan Evo 150 Liquid Handler
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