Koch Lab:Inventory/BRB80 (PEM): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 12: | Line 12: | ||
**12.5mM NaOH | **12.5mM NaOH | ||
*New batch made [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/08/28#PEM 8/28/2009] | *New batch made [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/08/28#PEM 8/28/2009] | ||
*Just over 200mL created | |||
**80mM PIPES | **80mM PIPES | ||
**1mM EGTA | **1mM EGTA | ||
Latest revision as of 11:20, 28 August 2009
BRB80 or PEM (PIPES, EGTA, Magnesium) buffer
Desired: 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, pH 6.9
- This is the page for the buffer that Andy, Brigette and Linh made on 19:07, 22 June 2009 (EDT)
- About 205ml were created\
- Concentrations:
- 1mM MgCl2
- 1mM EGTA
- 80mM PIPES
- 12.5mM NaOH
- New batch made 8/28/2009
- Just over 200mL created
- 80mM PIPES
- 1mM EGTA
- 1mM MgCl2
- 12.9mM NaOH
References
- Cytoskeleton PEM recipe
- The actual recipe Steve used at Sandia
- Recipe on kinesin home page (almost the same as cytoskeleton, but slightly lower pH)
- FriendFeed thread: what does the acronym BRB80 stand for?
