Koch Lab:Inventory/BRB80 (PEM): Difference between revisions
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**1mM EGTA | **1mM EGTA | ||
**80mM PIPES | **80mM PIPES | ||
** | **12.5mM NaOH | ||
*New batch made 8/28/2009 [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/08/28#PEM] | |||
**80mM PIPES | |||
**1mM EGTA | |||
**1mM MgCl<sub>2</sub> | |||
**12.9mM NaOH | |||
===References=== | ===References=== | ||
* [http://www.cytoskeleton.com/products/buffers/bst01.html Cytoskeleton PEM recipe] | * [http://www.cytoskeleton.com/products/buffers/bst01.html Cytoskeleton PEM recipe] |
Revision as of 11:19, 28 August 2009
BRB80 or PEM (PIPES, EGTA, Magnesium) buffer
Desired: 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, pH 6.9
- This is the page for the buffer that Andy, Brigette and Linh made on 19:07, 22 June 2009 (EDT)
- About 205ml were created\
- Concentrations:
- 1mM MgCl2
- 1mM EGTA
- 80mM PIPES
- 12.5mM NaOH
- New batch made 8/28/2009 [1]
- 80mM PIPES
- 1mM EGTA
- 1mM MgCl2
- 12.9mM NaOH
References
- Cytoskeleton PEM recipe
- The actual recipe Steve used at Sandia
- Recipe on kinesin home page (almost the same as cytoskeleton, but slightly lower pH)
- FriendFeed thread: what does the acronym BRB80 stand for?