Koch Lab:Protocols/Dig-bio PCR/pRL574 4411 PCR
5'-dig-GTAAAACGACGGCCAGTGAATTC (The Koch Lab calls this "pRL574-F853-dig")
- Should hybridize to the forward strand starting at basepair 853
5'-bio-GGAAACAGCTATGACCATGATTAC (The Koch Lab calls this "pRL574-R5263-bio")
- Should hyrbridize to the reverse strand starting at basepair 5263
It is not too expensive to purchase non-labeled primers to use as controls in case the PCR reaction doesn't work. Also sometimes it is convenient to perform PCR with only one label.