Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization/PEM-G

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Revision as of 10:33, 31 August 2009 by Andy Maloney (talk | contribs) (New page: '''PEM-G''' : 80 mM '''P'''IPES : 1 mM '''E'''GTA : 1 mM '''M'''gCl<sub>2</sub> : 1 mM '''G'''TP : ph 6.9 Cytoskeleton sells this in 2 parts. The first is [http://www.cytoskeleton.com/pr...)
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PEM-G

80 mM PIPES
1 mM EGTA
1 mM MgCl2
1 mM GTP
ph 6.9

Cytoskeleton sells this in 2 parts. The first is BST01 (PEM) and lyophilized GTP BST06 that you must resuspend in PEM to a concentration of 100 mM.

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