Koch Lab:Protocols/Loading rate clamp
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Calculation of instantaneous stiffness correction factor
Code documentation from early 2003
Here is the documentation from my LabVIEW sub-VI which calculates the "G" value, which is used in the loading rate clamp for unzipping DNA:
G is defined as "inverse of compliance per nm of ssDNA"...uh? say what?
G is basically stiffness multiplied by contour length, so that it has units of pN, and is independent of length of ssDNA.
The model is Smith, et al. FJC with stretch modulus.
I solved the equations by hand, but verified numerically that they are correct (to within a very small percentage, which is as careful as i was about numerical derivative)
I have added code so that it works above F = 5000 pN, but I'm not sure it's correct (who cares?)