Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex
From OpenWetWare
(Difference between revisions)
(New page: {{DNA Unzipping}}Category:Protocol {{Koch_Lab}} <div style="padding: 10px; width: 720px; border: 5px solid #008;"> </div>) |
|||
| Line 2: | Line 2: | ||
{{Koch_Lab}} | {{Koch_Lab}} | ||
<div style="padding: 10px; width: 720px; border: 5px solid #008;"> | <div style="padding: 10px; width: 720px; border: 5px solid #008;"> | ||
| + | ==Overview== | ||
| + | |||
| + | ==Materials== | ||
| + | |||
| + | ===Oligonucleotides=== | ||
| + | |||
| + | ===Oligo resuspension buffer=== | ||
| + | |||
| + | ===Oligo annealing buffer=== | ||
| + | |||
| + | |||
| + | ==Procedure== | ||
| + | |||
| + | ===Resuspend Oligos=== | ||
| + | |||
| + | ===Anneal duplexes=== | ||
| + | |||
| + | ==Storage and handling== | ||
| + | |||
| + | Store in 0.5 ml annealing tube. Freeze thaw cycles should not be an issue, but try not to bring too far above 4C when thawing. | ||
</div> | </div> | ||
Revision as of 15:01, 15 June 2008
| Home | Research | Lab Members | Publications | Protocols | Contact | Funding |
| Principles | Data | Notebooks | Links | Meetings | Presentations | Inventory |
