Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment: Difference between revisions
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This page describes the formation of the "anchoring segment" which is subsequently ligated to the insert oligo to create the base unzipping construct. | |||
==Materials== | |||
===Plasmid=== | |||
===Primers=== | |||
===PCR Materials=== | |||
===Restriction digest=== | |||
===Gel extraction=== | |||
==Procedure== | |||
</div> | </div> | ||
Revision as of 21:51, 14 June 2008
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This page describes the formation of the "anchoring segment" which is subsequently ligated to the insert oligo to create the base unzipping construct.
