Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment

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This page describes the formation of the "anchoring segment" which is subsequently ligated to the insert oligo to create the base unzipping construct.
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==Materials==
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===Plasmid===
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===Primers===
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===PCR Materials===
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===Restriction digest===
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===Gel extraction===
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==Procedure==
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Revision as of 23:51, 14 June 2008


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This page describes the formation of the "anchoring segment" which is subsequently ligated to the insert oligo to create the base unzipping construct.

Contents

Materials

Plasmid

Primers

PCR Materials

Restriction digest

Gel extraction

Procedure

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