Koeris/Notebook/2007-1-15: Difference between revisions

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=Pseudomonas aeruginosa mer operon cloning 3=
 
==Preparation==
What do i want to do in order to make sure the cloning works?
*Check concentrations of the chromatin DNA
*Do a dilution series of three orders of magnitude
==PCR of the operon==
#Master mix
##Template DNA equiv.
##ddH<sub>2</sub>O to 50uL
#Thermal profile
##92 deg C - 5min
##92 deg C - 30s
##50 deg C - 45s
##72 deg C - 120s
##4 deg C - indefinite
==Gel image==

Latest revision as of 07:23, 13 February 2007

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