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| =Pseudomonas aeruginosa mer operon cloning 3=
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| ==Preparation==
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| What can I do in order to make sure the cloning works this time?
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| *Check concentrations of the chromatin DNA: '''351 ng/ul'''
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| *Do a dilution series over two orders of magnitude of the template DNA: 3ng/ul - 300ng/ul
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| **All dilutions are to be done in at '''LEAST 100ul volume''' ddH<sub>2</sub>O
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| **Dilute stock 1:1.2: yields 300ng/ul
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| **Dilute twice 1:10: yields 30ng/ul and 3ng/ul
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| ==PCR set-up of the operon==
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| #Master mix
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| ##Template DNA equiv.
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| ##ddH<sub>2</sub>O to 50uL
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| #Thermal profile
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| ##92 deg C - 5min
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| ##92 deg C - 30s
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| ##50 deg C - 45s
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| ##72 deg C - 120s
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| ##4 deg C - indefinite
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| ===Pipetting scheme===
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| {| border="1"
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| ! Column heading 1 !! Column heading 2 !! Column heading 3
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| |-
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| ! Row heading 1
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| | Cell 2 || Cell 3
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| |-
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| ! Row heading A
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| |Cell B
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| |Cell C
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| |}
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| ==Gel image==
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| 1% agarose TAE gel, ran @ 100V for 60min
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