Koeris/Notebook/2007-1-15: Difference between revisions
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##72 deg C - 120s | ##72 deg C - 120s | ||
##4 deg C - indefinite | ##4 deg C - indefinite | ||
===Pipetting scheme=== | |||
{| border="1" | |||
! Column heading 1 !! Column heading 2 !! Column heading 3 | |||
|- | |||
! Row heading 1 | |||
| Cell 2 || Cell 3 | |||
|- | |||
! Row heading A | |||
|Cell B | |||
|Cell C | |||
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==Gel image== | ==Gel image== | ||
1% agarose TAE gel, ran @ 100V for 60min | 1% agarose TAE gel, ran @ 100V for 60min | ||
Revision as of 08:49, 15 January 2007
Pseudomonas aeruginosa mer operon cloning 3
Preparation
What can I do in order to make sure the cloning works this time?
- Check concentrations of the chromatin DNA
- Do a dilution series over three orders of magnitude of the template DNA
PCR set-up of the operon
- Master mix
- Template DNA equiv.
- ddH2O to 50uL
- Thermal profile
- 92 deg C - 5min
- 92 deg C - 30s
- 50 deg C - 45s
- 72 deg C - 120s
- 4 deg C - indefinite
Pipetting scheme
| Column heading 1 | Column heading 2 | Column heading 3 |
|---|---|---|
| Row heading 1 | Cell 2 | Cell 3 |
| Row heading A | Cell B | Cell C |
Gel image
1% agarose TAE gel, ran @ 100V for 60min
