Koeris/Notebook/2007-1-15

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Pseudomonas aeruginosa mer operon cloning 3

Preparation

What do i want to do in order to make sure the cloning works?

  • Check concentrations of the chromatin DNA
  • Do a dilution series of three orders of magnitude

PCR of the operon

  1. Master mix
    1. Template DNA equiv.
    2. ddH2O to 50uL
  2. Thermal profile
    1. 92 deg C - 5min
    2. 92 deg C - 30s
    3. 50 deg C - 45s
    4. 72 deg C - 120s
    5. 4 deg C - indefinite

Gel image

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