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| __TOC__
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| =Re-cloning of [His]<sub>6</sub>=
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| Due to lack of material, I could not recover the A plasmid and have to re-clone it.
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| ==PCR set-up==
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| *100 ul total reaction volume
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| *1ul template DNA @ ~40ng/ul
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| *200pmol primer mix
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| *97ul Taq SuperMix
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| ===Pipetting scheme===
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| Labels in the respective field codes
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| {| border="1"
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| ! Primer !! Tube Label
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| |-
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| ! A his6 2F&R 200pmol
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| | A1
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| |-
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| ! A his6 2F&R 200pmol
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| | A2
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| |}
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| ===Thermal profile===
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| #92 deg C - 5min
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| #92 deg C - 30s
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| #50 deg C - 45s
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| #72 deg C - 120s
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| #Cycle back to 2. 29X
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| #72 deg C - 10min
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| #4 deg C - indefinite
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