Koeris/Notebook/2007-1-21: Difference between revisions
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__TOC__ | __TOC__ | ||
=hipA assay= | =delta hipA assay= | ||
==Growth conditions== | ==Growth conditions== | ||
Grew all samples from previous 16h ON in | Grew all samples from previous 16h ON in | ||
Revision as of 09:23, 24 January 2007
delta hipA assay
Growth conditions
Grew all samples from previous 16h ON in
- 50ml LB + Cam
or
- 50ml M9 + 0.2% glucose + Cam
for another 16h, at 37 deg C and 300rpm shaking
Persister assay
Pipetting Scheme
| No antibiotic | No antibiotic | No antibiotic | No antibiotic | Kanamycin 30 ug/ml | Kanamycin 30 ug/ml |
|---|---|---|---|---|---|
| wt LB | wt M9 | DhipBA LB | DhipBA M9 | DhipA LB | DhipA M9 |
| Serial dilution | Serial dilution | Serial dilution | Serial dilution | Serial dilution | Serial dilution |
