Koeris/Notebook/2007-1-21

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(Pipetting Scheme)
Line 10: Line 10:
===Pipetting Scheme===
===Pipetting Scheme===
 +
{| border="1"
 +
! No antibiotic !! No antibiotic !! No antibiotic !! No antibiotic !! Kanamycin 30 ug/ml !! Kanamycin 30 ug/ml
 +
|-
 +
! wt LB !! wt M9 !! DhipBA LB !! DhipBA M9 !! DhipA LB !! DhipA M9
 +
|-
 +
! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution
 +
|-
 +
|}

Revision as of 12:21, 24 January 2007

Contents

hipA assay

Growth conditions

Grew all samples from previous 16h ON in

  • 50ml LB + Cam

or

  • 50ml M9 + 0.2% glucose + Cam

for another 16h, at 37 deg C and 300rpm shaking

Persister assay

Pipetting Scheme

No antibiotic No antibiotic No antibiotic No antibiotic Kanamycin 30 ug/ml Kanamycin 30 ug/ml
wt LB wt M9 DhipBA LB DhipBA M9 DhipA LB DhipA M9
Serial dilution Serial dilution Serial dilution Serial dilution Serial dilution Serial dilution
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