Koeris/Notebook/2007-1-21

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(hipA assay)
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__TOC__
 
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=delta hipA assay=
 
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==Growth conditions==
 
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Grew all samples from previous 16h ON in
 
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*50ml LB + Cam
 
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or
 
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*50ml M9 + 0.2% glucose + Cam
 
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for another 16h, at 37 deg C and 300rpm shaking
 
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==Persister assay==
 
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===Pipetting Scheme===
 
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{| border="1"
 
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! No antibiotic !! No antibiotic !! No antibiotic !! No antibiotic !! Kanamycin 30 ug/ml !! Kanamycin 30 ug/ml
 
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|-
 
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! wt LB !! wt M9 !! DhipBA LB !! DhipBA M9 !! DhipA LB !! DhipA M9
 
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|-
 
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! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution
 
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|-
 
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|}
 

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