Koeris/Notebook/2007-1-21: Difference between revisions

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__TOC__
=delta hipA assay=
==Growth conditions==
Grew all samples from previous 16h ON in
*50ml LB + Cam
or
*50ml M9 + 0.2% glucose + Cam
for another 16h, at 37 deg C and 300rpm shaking
==Persister assay==


===Pipetting Scheme===
{| border="1"
! No antibiotic !! No antibiotic !! No antibiotic !! No antibiotic !! Kanamycin 30 ug/ml !! Kanamycin 30 ug/ml
|-
! wt LB !! wt M9 !! DhipBA LB !! DhipBA M9 !! DhipA LB !! DhipA M9
|-
! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution !! Serial dilution
|-
|}

Latest revision as of 07:23, 13 February 2007

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