Koeris/Notebook/2007-1-23: Difference between revisions
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I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C. | I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C. | ||
==SDS-PAGE== | ==SDS-PAGE== | ||
We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12% | We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12% gels' and MES running buffer to get the high resolution in the low kDa-range (as advertised). | ||
Revision as of 12:19, 23 January 2007
Expression assay for [His]6 constructs
I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.
SDS-PAGE
We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12% gels' and MES running buffer to get the high resolution in the low kDa-range (as advertised).
