(Difference between revisions)
Revision as of 15:14, 23 January 2007
Expression assay for [His]6 constructs
I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.
We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12%