Koeris/Notebook/2007-1-23

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I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.
I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.
==SDS-PAGE==
==SDS-PAGE==
-
We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12%
+
We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12% gels' and MES running buffer to get the high resolution in the low kDa-range (as advertised).

Revision as of 15:19, 23 January 2007

Contents

Expression assay for [His]6 constructs

I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.

SDS-PAGE

We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12% gels' and MES running buffer to get the high resolution in the low kDa-range (as advertised).

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