Koeris/Notebook/2007-1-23: Difference between revisions
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=Expression assay for [His]<sub>6</sub> constructs= | =Expression assay for [His]<sub>6</sub> constructs= | ||
I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C. | I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C. | ||
Latest revision as of 14:36, 24 January 2007
Expression assay for [His]6 constructs
I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.