Kreeger:Research - Revision history

Kreeger at 15:46, 23 January 2013

Kreeger — Wed, 23 Jan 2013 15:46:08 GMT

←Older revision		Revision as of 15:46, 23 January 2013
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	'''Analysis of Cell-Cell Interactions in Endometriosis'''<br>		'''Analysis of Cell-Cell Interactions in Endometriosis'''<br>

-	Endometriosis is a chronic gynecological condition that affects 5-10% of women resulting in pelvic pain and infertility. In endometriosis, endometrial tissue is found outside of the uterus, attached to the ovaries, uterine ligaments, and virtually any other region of the body. This ectopic endometrium triggers local inflammation in response to cyclic changes in estrogen, resulting in severe pain. Despite its prevalence and significant impact on women’s health, relatively little is known about how the disease develops or how to intervene due to a lack of model systems. We are developing an in vitro model that incorporates the epithelial, stromal, and macrophage components of the disease in order to examine how cell-cell interactions impact disease progression. Using this system, we ~~hope to determine which~~ interactions drive endometriosis and identify therapeutic targets.<br>	+	Endometriosis is a chronic gynecological condition that affects 5-10% of women resulting in pelvic pain and infertility. In endometriosis, endometrial tissue is found outside of the uterus, attached to the ovaries, uterine ligaments, and virtually any other region of the body. This ectopic endometrium triggers local inflammation in response to cyclic changes in estrogen, resulting in severe pain. Despite its prevalence and significant impact on women’s health, relatively little is known about how the disease develops or how to intervene due to a lack of model systems. We are developing an in vitro model that incorporates the epithelial, stromal, and macrophage components of the disease in order to examine how cell-cell interactions impact disease progression. Using this system, we will examine interactions drive endometriosis and, in collaboration with [http://obgyn.wisc.edu/directory/detail.aspx?id=19 Dr. Dan Lebovic], identify therapeutic targets.<br>
	<br>		<br>
	'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>		'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>

Kreeger at 15:44, 23 January 2013

Kreeger — Wed, 23 Jan 2013 15:44:11 GMT

←Older revision		Revision as of 15:44, 23 January 2013
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	'''Cellular and Signaling Network Cross-Talk in Ovarian Cancer''' <br>		'''Cellular and Signaling Network Cross-Talk in Ovarian Cancer''' <br>
	[[Image:OVCA_project_overview.jpg\|350px\|right]]		[[Image:OVCA_project_overview.jpg\|350px\|right]]
-	Ovarian cancer has a mortality rate of greater than 50%, primarily due to the late stage at which it is diagnosed. This late diagnosis complicates treatment - patients accumulate different mutations in their tumors and tumor cells receive a variety of stimuli from other cell types during disease progression, making it impossible to define a blanket treatment for everyone. We are working to address this complex scenario through several complementary approaches. First, we are characterizing a panel of ovarian cancer cell lines to capture this diversity and determine how cells respond to current drugs. By delineating molecular signatures that correspond to drug sensitivities we hope to better match patients to drugs and improve prognosis. Secondly, we are examining cross-talk between signaling pathways in the tumor cell to determine signals that are responsible for controlling proliferation, providing new drug targets for ovarian cancer. Finally, we are developing in vitro culture systems to study interactions between ovarian cancer tumor cells and other cells in the tumor microenvironment in order to identify new approaches to control tumor growth. <br>	+	Ovarian cancer has a mortality rate of greater than 50%, primarily due to the late stage at which it is diagnosed. This late diagnosis complicates treatment - patients accumulate different mutations in their tumors and tumor cells receive a variety of stimuli from other cell types during disease progression, making it impossible to define a blanket treatment for everyone. We are working to address this complex scenario through several complementary approaches. First, we are characterizing a panel of ovarian cancer cell lines to capture this diversity and determine how cells respond to current drugs. By delineating molecular signatures that correspond to drug sensitivities we hope to better match patients to drugs and improve prognosis. Secondly, we are examining cross-talk between signaling pathways in the tumor cell to determine signals that are responsible for controlling proliferation, providing new drug targets for ovarian cancer. Finally, in collaboration with [http://obgyn.wisc.edu/directory/detail.aspx?id=40 Prof. Manish Patankar] we are developing in vitro culture systems to study interactions between ovarian cancer tumor cells and other cells in the tumor microenvironment in order to identify new approaches to control tumor growth. <br>
	<br>		<br>
	[[Image:KreegerEndo.jpg\|200px\|right]]		[[Image:KreegerEndo.jpg\|200px\|right]]

Kreeger at 18:39, 23 October 2012

Kreeger — Tue, 23 Oct 2012 18:39:14 GMT

←Older revision		Revision as of 18:39, 23 October 2012
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	<br>		<br>
	'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>		'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>
-	Tissue engineers are working to develop methods to restore or improve tissue function, largely through a process of trial and error. In contrast, we are working to determine design principles to inform the creation of tissue engineering scaffolds. In collaboration with [http://~~kristynmasters~~.~~com~~/ Prof. Kristyn Masters] and [http://nitrolab.engr.wisc.edu/ Prof. Justin Williams], we are characterizing how keratinocytes and endothelial cells interpret, integrate, and respond to simple and complex combinations of microenvironmental cues. In particular, we are examining the link between environmental cues, the downstream signaling events that result, and cellular responses. By identifying this relationship, we will be able to identify combinations of stimuli that promote desirable behaviors such as increased migration for wound healing or increased tubule formation for angiogenesis.<br>	+	Tissue engineers are working to develop methods to restore or improve tissue function, largely through a process of trial and error. In contrast, we are working to determine design principles to inform the creation of tissue engineering scaffolds. In collaboration with [http://openwetware.org/wiki/Masters Prof. Kristyn Masters] and [http://nitrolab.engr.wisc.edu/ Prof. Justin Williams], we are characterizing how keratinocytes and endothelial cells interpret, integrate, and respond to simple and complex combinations of microenvironmental cues. In particular, we are examining the link between environmental cues, the downstream signaling events that result, and cellular responses. By identifying this relationship, we will be able to identify combinations of stimuli that promote desirable behaviors such as increased migration for wound healing or increased tubule formation for angiogenesis.<br>

	<br>		<br>

Kreeger at 18:36, 23 October 2012

Kreeger — Tue, 23 Oct 2012 18:36:43 GMT

←Older revision		Revision as of 18:36, 23 October 2012
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	<br>		<br>
	'''Funding''' <br>		'''Funding''' <br>
-	~~Funding for our research is provided by:<br>~~
	NSF CAREER award<br>		NSF CAREER award<br>
	NIH/NIGMS R01 (PI: KS Masters)<br>		NIH/NIGMS R01 (PI: KS Masters)<br>

Kreeger at 18:36, 23 October 2012

Kreeger — Tue, 23 Oct 2012 18:36:22 GMT

←Older revision		Revision as of 18:36, 23 October 2012
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	NSF CAREER award<br>		NSF CAREER award<br>
	NIH/NIGMS R01 (PI: KS Masters)<br>		NIH/NIGMS R01 (PI: KS Masters)<br>
-	American Cancer Society ~~Institutional~~ Research Grant<br>	+	American Cancer Society Research Scholars Grant<br>
	UW-Madison Graduate School<br>		UW-Madison Graduate School<br>
	Thank you to [http://www.turnerbiosystems.com/ Turner Biosystems] for granting us a Veritas Microplate Luminometer		Thank you to [http://www.turnerbiosystems.com/ Turner Biosystems] for granting us a Veritas Microplate Luminometer

Kreeger at 14:46, 24 January 2012

Kreeger — Tue, 24 Jan 2012 14:46:28 GMT

←Older revision		Revision as of 14:46, 24 January 2012
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	<br>		<br>
	'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>		'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>
-	Tissue ~~engineering aims~~ to develop methods to restore or improve tissue function~~. To date~~, ~~tissue engineering strategies have been~~ largely a ~~matter~~ of trial and error. In contrast, we are working to determine design principles to inform the creation of tissue engineering scaffolds. In collaboration with [http://kristynmasters.com/ Prof. Kristyn Masters] and [http://nitrolab.engr.wisc.edu/ Prof. Justin Williams], we are ~~working to characterize~~ how keratinocytes and endothelial cells interpret, integrate, and respond to simple and complex combinations of microenvironmental cues. In particular, we are examining the link between environmental cues, the downstream signaling events that result, and cellular responses. By identifying this relationship, we will be able to identify combinations of stimuli that promote desirable behaviors such as increased migration for wound healing or increased tubule formation for angiogenesis.<br>	+	Tissue engineers are working to develop methods to restore or improve tissue function, largely through a process of trial and error. In contrast, we are working to determine design principles to inform the creation of tissue engineering scaffolds. In collaboration with [http://kristynmasters.com/ Prof. Kristyn Masters] and [http://nitrolab.engr.wisc.edu/ Prof. Justin Williams], we are characterizing how keratinocytes and endothelial cells interpret, integrate, and respond to simple and complex combinations of microenvironmental cues. In particular, we are examining the link between environmental cues, the downstream signaling events that result, and cellular responses. By identifying this relationship, we will be able to identify combinations of stimuli that promote desirable behaviors such as increased migration for wound healing or increased tubule formation for angiogenesis.<br>

	<br>		<br>

Kreeger at 04:16, 24 January 2012

Kreeger — Tue, 24 Jan 2012 04:16:38 GMT

←Older revision		Revision as of 04:16, 24 January 2012
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	<br>		<br>
	[[Image:KreegerEndo.jpg\|200px\|right]]		[[Image:KreegerEndo.jpg\|200px\|right]]
-	'''~~Bioengineering~~ Analysis of Cell-Cell Interactions in Endometriosis'''<br>	+	'''Analysis of Cell-Cell Interactions in Endometriosis'''<br>

	Endometriosis is a chronic gynecological condition that affects 5-10% of women resulting in pelvic pain and infertility. In endometriosis, endometrial tissue is found outside of the uterus, attached to the ovaries, uterine ligaments, and virtually any other region of the body. This ectopic endometrium triggers local inflammation in response to cyclic changes in estrogen, resulting in severe pain. Despite its prevalence and significant impact on women’s health, relatively little is known about how the disease develops or how to intervene due to a lack of model systems. We are developing an in vitro model that incorporates the epithelial, stromal, and macrophage components of the disease in order to examine how cell-cell interactions impact disease progression. Using this system, we hope to determine which interactions drive endometriosis and identify therapeutic targets.<br>		Endometriosis is a chronic gynecological condition that affects 5-10% of women resulting in pelvic pain and infertility. In endometriosis, endometrial tissue is found outside of the uterus, attached to the ovaries, uterine ligaments, and virtually any other region of the body. This ectopic endometrium triggers local inflammation in response to cyclic changes in estrogen, resulting in severe pain. Despite its prevalence and significant impact on women’s health, relatively little is known about how the disease develops or how to intervene due to a lack of model systems. We are developing an in vitro model that incorporates the epithelial, stromal, and macrophage components of the disease in order to examine how cell-cell interactions impact disease progression. Using this system, we hope to determine which interactions drive endometriosis and identify therapeutic targets.<br>

Kreeger at 04:16, 24 January 2012

Kreeger — Tue, 24 Jan 2012 04:16:05 GMT

←Older revision		Revision as of 04:16, 24 January 2012
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	Ovarian cancer has a mortality rate of greater than 50%, primarily due to the late stage at which it is diagnosed. This late diagnosis complicates treatment - patients accumulate different mutations in their tumors and tumor cells receive a variety of stimuli from other cell types during disease progression, making it impossible to define a blanket treatment for everyone. We are working to address this complex scenario through several complementary approaches. First, we are characterizing a panel of ovarian cancer cell lines to capture this diversity and determine how cells respond to current drugs. By delineating molecular signatures that correspond to drug sensitivities we hope to better match patients to drugs and improve prognosis. Secondly, we are examining cross-talk between signaling pathways in the tumor cell to determine signals that are responsible for controlling proliferation, providing new drug targets for ovarian cancer. Finally, we are developing in vitro culture systems to study interactions between ovarian cancer tumor cells and other cells in the tumor microenvironment in order to identify new approaches to control tumor growth. <br>		Ovarian cancer has a mortality rate of greater than 50%, primarily due to the late stage at which it is diagnosed. This late diagnosis complicates treatment - patients accumulate different mutations in their tumors and tumor cells receive a variety of stimuli from other cell types during disease progression, making it impossible to define a blanket treatment for everyone. We are working to address this complex scenario through several complementary approaches. First, we are characterizing a panel of ovarian cancer cell lines to capture this diversity and determine how cells respond to current drugs. By delineating molecular signatures that correspond to drug sensitivities we hope to better match patients to drugs and improve prognosis. Secondly, we are examining cross-talk between signaling pathways in the tumor cell to determine signals that are responsible for controlling proliferation, providing new drug targets for ovarian cancer. Finally, we are developing in vitro culture systems to study interactions between ovarian cancer tumor cells and other cells in the tumor microenvironment in order to identify new approaches to control tumor growth. <br>
	<br>		<br>
-	~~'''Bioengineering Analysis of Cell-Cell Interactions in Endometriosis'''<br>~~
	[[Image:KreegerEndo.jpg\|200px\|right]]		[[Image:KreegerEndo.jpg\|200px\|right]]
		+	'''Bioengineering Analysis of Cell-Cell Interactions in Endometriosis'''<br>
		+
	Endometriosis is a chronic gynecological condition that affects 5-10% of women resulting in pelvic pain and infertility. In endometriosis, endometrial tissue is found outside of the uterus, attached to the ovaries, uterine ligaments, and virtually any other region of the body. This ectopic endometrium triggers local inflammation in response to cyclic changes in estrogen, resulting in severe pain. Despite its prevalence and significant impact on women’s health, relatively little is known about how the disease develops or how to intervene due to a lack of model systems. We are developing an in vitro model that incorporates the epithelial, stromal, and macrophage components of the disease in order to examine how cell-cell interactions impact disease progression. Using this system, we hope to determine which interactions drive endometriosis and identify therapeutic targets.<br>		Endometriosis is a chronic gynecological condition that affects 5-10% of women resulting in pelvic pain and infertility. In endometriosis, endometrial tissue is found outside of the uterus, attached to the ovaries, uterine ligaments, and virtually any other region of the body. This ectopic endometrium triggers local inflammation in response to cyclic changes in estrogen, resulting in severe pain. Despite its prevalence and significant impact on women’s health, relatively little is known about how the disease develops or how to intervene due to a lack of model systems. We are developing an in vitro model that incorporates the epithelial, stromal, and macrophage components of the disease in order to examine how cell-cell interactions impact disease progression. Using this system, we hope to determine which interactions drive endometriosis and identify therapeutic targets.<br>
	<br>		<br>

Kreeger at 04:13, 24 January 2012

Kreeger — Tue, 24 Jan 2012 04:13:40 GMT

←Older revision		Revision as of 04:13, 24 January 2012
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	<br>		<br>
	'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>		'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>
-	Tissue engineering aims to develop methods to restore or improve tissue function. To date, tissue engineering strategies have been largely a matter of trial and error. In contrast, we are working to determine design principles to inform the creation of tissue engineering scaffolds. In collaboration with Prof. Kristyn Masters and Prof. Justin Williams, we are working to characterize how keratinocytes and endothelial cells interpret, integrate, and respond to simple and complex combinations of microenvironmental cues. In particular, we are examining the link between environmental cues, the downstream signaling events that result, and cellular responses. By identifying this relationship, we will be able to identify combinations of stimuli that promote desirable behaviors such as increased migration for wound healing or increased tubule formation for angiogenesis.<br>	+	Tissue engineering aims to develop methods to restore or improve tissue function. To date, tissue engineering strategies have been largely a matter of trial and error. In contrast, we are working to determine design principles to inform the creation of tissue engineering scaffolds. In collaboration with [http://kristynmasters.com/ Prof. Kristyn Masters] and [http://nitrolab.engr.wisc.edu/ Prof. Justin Williams], we are working to characterize how keratinocytes and endothelial cells interpret, integrate, and respond to simple and complex combinations of microenvironmental cues. In particular, we are examining the link between environmental cues, the downstream signaling events that result, and cellular responses. By identifying this relationship, we will be able to identify combinations of stimuli that promote desirable behaviors such as increased migration for wound healing or increased tubule formation for angiogenesis.<br>

	<br>		<br>

Kreeger at 04:12, 24 January 2012

Kreeger — Tue, 24 Jan 2012 04:12:15 GMT

←Older revision		Revision as of 04:12, 24 January 2012
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	<br>		<br>
	'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>		'''Cellular Decision-Making Processes in Wound Healing and Angiogenesis''' <br>
-	~~In both in vivo and in vitro environments, cells are exposed~~ to ~~numerous extracellular stimuli that regulate their~~ function~~, such as extracellular matrix proteins, growth factors, and mechanical forces~~. ~~The nature~~ of ~~these cues~~ and ~~their subsequent interpretation by cells is complex; sometimes these cues work in concert~~ to ~~instruct cells~~ to ~~perform a certain function,~~ and ~~other times these cues are conflicting~~. ~~We aim~~ to ~~better understand~~ how cells ~~“decide” what cues~~ to ~~follow in this~~ complex ~~environment~~, ~~particularly in~~ the ~~context of dermal wound healing~~ and ~~angiogenesis~~. ~~This work~~ will ~~also~~ be ~~combined with computational modeling (collaboration with Prof. Pam Kreeger)~~ to ~~predict cell behavior in untested environments. Such efforts to better understand and predict cellular decision-making processes is important in not only informing the construction~~ of ~~environments~~ that ~~allow greater control over cell behavior, but also in understanding native physiological phenomena~~.<br>	+	Tissue engineering aims to develop methods to restore or improve tissue function. To date, tissue engineering strategies have been largely a matter of trial and error. In contrast, we are working to determine design principles to inform the creation of tissue engineering scaffolds. In collaboration with Prof. Kristyn Masters and Prof. Justin Williams, we are working to characterize how keratinocytes and endothelial cells interpret, integrate, and respond to simple and complex combinations of microenvironmental cues. In particular, we are examining the link between environmental cues, the downstream signaling events that result, and cellular responses. By identifying this relationship, we will be able to identify combinations of stimuli that promote desirable behaviors such as increased migration for wound healing or increased tubule formation for angiogenesis.<br>

	<br>		<br>