http://www.openwetware.org/index.php?title=Kreitman:Research&feed=atom&action=historyKreitman:Research - Revision history2013-05-20T17:51:29ZRevision history for this page on the wikiMediaWiki 1.13.2http://www.openwetware.org/index.php?title=Kreitman:Research&diff=499506&oldid=prevCecelia M. Miles: /* Egg size variation */2011-03-17T18:13:34Z<p><span class="autocomment">Egg size variation</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We are using several techniques to visualize the way that different selective pressures on egg size affect the spatial patterning of early segmentation genes in developing ''Drosophila'' embryos. We have used artificial selection to generate replicate large and small egg lines. Using ''in situ'' hybridization, we triple stained these embryos for mRNA of ''giant'' (''gt''), and ''even-skipped'' (''eve''), with Sytox green as a nuclear stain. We used some innovative software called PointCloudToolbox (http://bdtnp.lbl.gov/Fly-Net/bioimaging.jsp?w=software) to compare the ''eve'' stripe patterns between the divergently selected lines, as well as number of nuclei at the periphery for mitotic cycle 14 embryos. We found that there is genetic variation for ''eve'' stripe allometry segregating in natural populations of ''D. melanogaster''. The phenotypic difference in stripe positioning between the selected lines is neither a spatially restricted shift, nor a coordinate shift of all stripes, but a monotonically increasing (i.e., allometric) shift along both A-P and D-V axes (Figure <del class="diffchange diffchange-inline">1</del>). Genetic variation for this buffered trait was produced in the absence of environmental or genetic perturbation, and therefore must not be entirely suppressed by the developmental canalization process. Also, cycle 14 nuclear number is not constant, but differs by a mean of more than 1000 nuclei as a correlated response to divergent selection for egg size. In addition, we have generated replicate ''Drosophila'' lines (from the same base population as the selected lines) with evolved differences in egg size as a result of reducing the temperature in which they were raised. Analysis of these lines is ongoing. We derived inbred sublines from the artificially selected populations <del class="diffchange diffchange-inline">and extracted RNA from ovaries dissected from these females. We plan to use resequencing techniques to compare expression between the divergently selected lines </del>and we are currently surveying these inbred sublines for ''eve'' stripe border position.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We are using several techniques to visualize the way that different selective pressures on egg size affect the spatial patterning of early segmentation genes in developing ''Drosophila'' embryos. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:Fig2_.png|thumb|right|Three dimensional representation of stripe border displacement]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We have used artificial selection to generate replicate large and small egg lines. Using ''in situ'' hybridization, we triple stained these embryos for mRNA of ''giant'' (''gt''), and ''even-skipped'' (''eve''), with Sytox green as a nuclear stain. We used some innovative software called PointCloudToolbox (http://bdtnp.lbl.gov/Fly-Net/bioimaging.jsp?w=software) to compare the ''eve'' stripe patterns between the divergently selected lines, as well as number of nuclei at the periphery for mitotic cycle 14 embryos. We found that there is genetic variation for ''eve'' stripe allometry segregating in natural populations of ''D. melanogaster''. The phenotypic difference in stripe positioning between the selected lines is neither a spatially restricted shift, nor a coordinate shift of all stripes, but a monotonically increasing (i.e., allometric) shift along both A-P and D-V axes (Figure). Genetic variation for this buffered trait was produced in the absence of environmental or genetic perturbation, and therefore must not be entirely suppressed by the developmental canalization process. Also, cycle 14 nuclear number is not constant, but differs by a mean of more than 1000 nuclei as a correlated response to divergent selection for egg size. In addition, we have generated replicate ''Drosophila'' lines (from the same base population as the selected lines) with evolved differences in egg size as a result of reducing the temperature in which they were raised. Analysis of these lines is ongoing. We derived inbred sublines from the artificially selected populations and we are currently surveying these inbred sublines for ''eve'' stripe border position <ins class="diffchange diffchange-inline">along with several gap gene expression domains</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td></tr>
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</table>Cecelia M. Mileshttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499505&oldid=prevCecelia M. Miles: /* Egg size variation */2011-03-17T18:07:15Z<p><span class="autocomment">Egg size variation</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:07, 17 March 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We are using several techniques to visualize the way that different selective pressures on egg size affect the spatial patterning of early segmentation genes in developing ''Drosophila'' embryos. We have used artificial selection to generate replicate large and small egg lines. Using ''in situ'' hybridization, we triple stained these embryos for mRNA of ''giant'' (''gt''), and ''even-skipped'' (''eve''), with Sytox green as a nuclear stain. We used some innovative software called PointCloudToolbox (http://bdtnp.lbl.gov/Fly-Net/bioimaging.jsp?w=software) to compare the ''eve'' stripe patterns between the divergently selected lines, as well as number of nuclei at the periphery for mitotic cycle 14 embryos. <del class="diffchange diffchange-inline">This manuscript </del>is <del class="diffchange diffchange-inline">online early </del>in the <del class="diffchange diffchange-inline">journal Evolution </del>(<del class="diffchange diffchange-inline">DOI:10</del>.<del class="diffchange diffchange-inline">1111/j</del>.<del class="diffchange diffchange-inline">1558</del>-<del class="diffchange diffchange-inline">5646</del>.<del class="diffchange diffchange-inline">2010</del>.<del class="diffchange diffchange-inline">01088</del>.<del class="diffchange diffchange-inline">x). We </del>have <del class="diffchange diffchange-inline">also </del>generated replicate ''Drosophila'' lines (from the same base population as the selected lines) with evolved differences in egg size as a result of reducing the temperature in which they were raised. Analysis of these lines is ongoing. We derived inbred sublines from the artificially selected populations and extracted RNA from ovaries dissected from these females. We plan to use resequencing techniques to compare expression between the divergently selected lines and we are currently surveying these inbred sublines for ''eve'' stripe border position.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We are using several techniques to visualize the way that different selective pressures on egg size affect the spatial patterning of early segmentation genes in developing ''Drosophila'' embryos. We have used artificial selection to generate replicate large and small egg lines. Using ''in situ'' hybridization, we triple stained these embryos for mRNA of ''giant'' (''gt''), and ''even-skipped'' (''eve''), with Sytox green as a nuclear stain. We used some innovative software called PointCloudToolbox (http://bdtnp.lbl.gov/Fly-Net/bioimaging.jsp?w=software) to compare the ''eve'' stripe patterns between the divergently selected lines, as well as number of nuclei at the periphery for mitotic cycle 14 embryos. <ins class="diffchange diffchange-inline">We found that there </ins>is <ins class="diffchange diffchange-inline">genetic variation for ''eve'' stripe allometry segregating </ins>in <ins class="diffchange diffchange-inline">natural populations of ''D. melanogaster''. The phenotypic difference in stripe positioning between </ins>the <ins class="diffchange diffchange-inline">selected lines is neither a spatially restricted shift, nor a coordinate shift of all stripes, but a monotonically increasing </ins>(<ins class="diffchange diffchange-inline">i</ins>.<ins class="diffchange diffchange-inline">e</ins>.<ins class="diffchange diffchange-inline">, allometric) shift along both A</ins>-<ins class="diffchange diffchange-inline">P and D-V axes (Figure 1)</ins>. <ins class="diffchange diffchange-inline">Genetic variation for this buffered trait was produced in the absence of environmental or genetic perturbation, and therefore must not be entirely suppressed by the developmental canalization process</ins>. <ins class="diffchange diffchange-inline"> Also, cycle 14 nuclear number is not constant, but differs by a mean of more than 1000 nuclei as a correlated response to divergent selection for egg size</ins>. <ins class="diffchange diffchange-inline"> In addition, we </ins>have generated replicate ''Drosophila'' lines (from the same base population as the selected lines) with evolved differences in egg size as a result of reducing the temperature in which they were raised. Analysis of these lines is ongoing. We derived inbred sublines from the artificially selected populations and extracted RNA from ovaries dissected from these females. We plan to use resequencing techniques to compare expression between the divergently selected lines and we are currently surveying these inbred sublines for ''eve'' stripe border position.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td></tr>
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</table>Cecelia M. Mileshttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499499&oldid=prevBin He: /* Drosophila Transcriptional Enhancers Evolution */2011-03-17T16:54:32Z<p><span class="autocomment">Drosophila Transcriptional Enhancers Evolution</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:MK ratio comb2.png|thumb|right|Ratio of Divergence to Polymorphism]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:MK ratio comb2.png|thumb|right|Ratio of Divergence to Polymorphism <ins class="diffchange diffchange-inline">Suggesting Positive Selection</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td></tr>
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</table>Bin Hehttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499498&oldid=prevBin He: /* Drosophila Transcriptional Enhancers Evolution */2011-03-17T16:53:58Z<p><span class="autocomment">Drosophila Transcriptional Enhancers Evolution</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:MK ratio comb2.png|thumb|right|Ratio of Divergence to Polymorphism]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:MK ratio comb2.png|thumb|right|Ratio of Divergence to Polymorphism]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td></tr>
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</table>Bin Hehttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499497&oldid=prevBin He: /* Drosophila Transcriptional Enhancers Evolution */2011-03-17T16:53:41Z<p><span class="autocomment">Drosophila Transcriptional Enhancers Evolution</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:MK ratio comb2.png|thumb|right|Ratio of Divergence to Polymorphism]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:MK ratio comb2.png|thumb|right|Ratio of Divergence to Polymorphism]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td></tr>
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</table>Bin Hehttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499496&oldid=prevBin He: /* Drosophila Transcriptional Enhancers Evolution */2011-03-17T16:53:11Z<p><span class="autocomment">Drosophila Transcriptional Enhancers Evolution</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:MK ratio comb2.png|thumb|right|Ratio of Divergence to Polymorphism]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. This finding challenges the prevailing neutral view of the TFBS turnover process, indicating that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. However, the net change in CRM function will be highly constrained due to pleiotropic effects and essentiality of the gene function, resulting in a distinct short term vs. long term evolutionary dynamics.</div></td></tr>
<!-- diff generator: internal 2013-05-20 17:51:29 -->
</table>Bin Hehttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499488&oldid=prevBin He: /* Drosophila Transcriptional Enhancers Evolution */2011-03-17T16:43:21Z<p><span class="autocomment">Drosophila Transcriptional Enhancers Evolution</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">Revision as of 16:43, 17 March 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral<del class="diffchange diffchange-inline">. To the contrary, genome-wide population genetics analyses have unequivocally identified signatures of positive selection acting in noncoding regions in general, and particularly in 5' and 3' untranscribed regions of genes</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. <del class="diffchange diffchange-inline">Spacer sequences also showed signatures </del>of <del class="diffchange diffchange-inline">negative and positive selection. Based on these results</del>, <del class="diffchange diffchange-inline">we propose </del>that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. <del class="diffchange diffchange-inline">In the mean time</del>, <del class="diffchange diffchange-inline">however, the high connectivity in the regulatory network implies pleiotropic effects while the essentiality of genes controlled by the network may call for accurate regulation, both suggesting that </del>the net change in CRM function will be highly constrained, <del class="diffchange diffchange-inline">which would explain why distantly related species with their CRM sequences diverged beyond recognition could still drive highly similar expression patterns </del>in a <del class="diffchange diffchange-inline">transgenic reporter assay</del>. <del class="diffchange diffchange-inline">In the future, we are interested in elucidating the functional consequences of TFBS turnover and the relevant co-evolution in the trans or other cis components to better understand how gene regulation evolves and how they contribute to species divergence and/or phenotypic evolution</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. <ins class="diffchange diffchange-inline">This finding challenges the prevailing neutral view </ins>of <ins class="diffchange diffchange-inline">the TFBS turnover process</ins>, <ins class="diffchange diffchange-inline">indicating </ins>that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. <ins class="diffchange diffchange-inline">However</ins>, the net change in CRM function will be highly constrained <ins class="diffchange diffchange-inline">due to pleiotropic effects and essentiality of the gene function</ins>, <ins class="diffchange diffchange-inline">resulting </ins>in a <ins class="diffchange diffchange-inline">distinct short term vs</ins>. <ins class="diffchange diffchange-inline">long term evolutionary dynamics</ins>.</div></td></tr>
<!-- diff generator: internal 2013-05-20 17:51:29 -->
</table>Bin Hehttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499487&oldid=prevBin He: /* Drosophila Transcriptional Enhancers Evolution */2011-03-17T16:37:43Z<p><span class="autocomment">Drosophila Transcriptional Enhancers Evolution</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 16:37, 17 March 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Drosophila Transcriptional Enhancers Evolution==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral. To the contrary, genome-wide population genetics analyses have unequivocally identified signatures of positive selection acting in noncoding regions in general, and particularly in 5' and 3' untranscribed regions of genes. To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. Spacer sequences also showed signatures of negative and positive selection. Based on these results, we propose that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. In the mean time, however, the high connectivity in the regulatory network implies pleiotropic effects while the essentiality of genes controlled by the network may call for accurate regulation, both suggesting that the net change in CRM function will be highly constrained, which would explain why distantly related species with their CRM sequences diverged beyond recognition could still drive highly similar expression patterns in a transgenic reporter assay. In the future, we are interested in elucidating the functional consequences of TFBS turnover and the relevant co-evolution in the trans or other cis components to better understand how gene regulation evolves and how they contribute to species divergence and/or phenotypic evolution.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Transcription factor binding sites (TFBS) turnover (i.e. lineage specific gain and loss) is a well-documented phenomenon in eukaryote cis-regulatory modules (CRM). The wide spread of the phenomenon and the appearance of conserved expression patterns for diverged orthologous CRM led to the standing view that the observed gain and loss of TFBS were functionally and selectively neutral. To the contrary, genome-wide population genetics analyses have unequivocally identified signatures of positive selection acting in noncoding regions in general, and particularly in 5' and 3' untranscribed regions of genes. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To specifically test the neutral vs. selection hypotheses for the TFBS turnover process, we analyzed natural variation patterns within and between two closely related ''Drosophila'' species in a set of experimentally defined and validated TFBS dataset (http://redfly.ccr.buffalo.edu/). We found the patterns of divergence and polymorphism for two types of mutations -- those inferred to increase or decrease the binding affinity respectively-- are not compatible with a neutral hypothesis. Instead, multiple lines of evidence suggested that positive selection have contributed to gain as well as loss of TFBS in the two lineages, with purifying selection maintaining existing TFBS in the population. Spacer sequences also showed signatures of negative and positive selection. Based on these results, we propose that CRM function may experience constant pressures to adapt to both external environmental changes as well as internal genetic network alterations. In the mean time, however, the high connectivity in the regulatory network implies pleiotropic effects while the essentiality of genes controlled by the network may call for accurate regulation, both suggesting that the net change in CRM function will be highly constrained, which would explain why distantly related species with their CRM sequences diverged beyond recognition could still drive highly similar expression patterns in a transgenic reporter assay. In the future, we are interested in elucidating the functional consequences of TFBS turnover and the relevant co-evolution in the trans or other cis components to better understand how gene regulation evolves and how they contribute to species divergence and/or phenotypic evolution.</div></td></tr>
<!-- diff generator: internal 2013-05-20 17:51:29 -->
</table>Bin Hehttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499486&oldid=prevBin He: /* Molecular population genetics and evolution (Drosophila and Arabidopsis) */2011-03-17T16:35:44Z<p><span class="autocomment">Molecular population genetics and evolution (Drosophila and Arabidopsis)</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 16:35, 17 March 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Molecular population genetics and evolution (Drosophila and Arabidopsis)==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Molecular population genetics and evolution (Drosophila and Arabidopsis)==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>I have had a longstanding interest in molecular population genetics, and have contributed over the years to the development of methods for analyzing DNA sequence polymorphism and evolutionary data, particularly with respect to detecting the action of natural selection. Although our work has historically focused on Drosophila, we have shifted our attention in recent years to Arabidopsis thaliana and its bacterial pathogens (in collaboration with Joy Bergelson's lab). Plant resistance to bacterial disease and bacterial pathenogenicity is an immensely powerful system for investigating both the ecological and evolutionary contexts of molecular adaptation, and holds the promise of uniting the two. In collaboration with Magnus Nordborg (http://walnut.usc.edu/) and Justin Borevitz, we have completed genome-wide assessments of genetic polymorphism, have assembled at 250K SNP chip, and have genotyped hundreds of isolines derived from natural populations. We have validated genome-wide association mapping in Arabidopsis to discover disease resistance polymorphism, and intend to do the same with common bacterial pathogens. We are also carrying out metagenomic surveys of the bacterial pathogen community infecting Arabidopsis.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>I have had a longstanding interest in molecular population genetics, and have contributed over the years to the development of methods for analyzing DNA sequence polymorphism and evolutionary data, particularly with respect to detecting the action of natural selection. Although our work has historically focused on Drosophila, we have shifted our attention in recent years to Arabidopsis thaliana and its bacterial pathogens (in collaboration with Joy Bergelson's lab). Plant resistance to bacterial disease and bacterial pathenogenicity is an immensely powerful system for investigating both the ecological and evolutionary contexts of molecular adaptation, and holds the promise of uniting the two. In collaboration with Magnus Nordborg (http://walnut.usc.edu/) and Justin Borevitz, we have completed genome-wide assessments of genetic polymorphism, have assembled at 250K SNP chip, and have genotyped hundreds of isolines derived from natural populations. We have validated genome-wide association mapping in Arabidopsis to discover disease resistance polymorphism, and intend to do the same with common bacterial pathogens. We are also carrying out metagenomic surveys of the bacterial pathogen community infecting Arabidopsis.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>Ever since our early studies of sequence variation and evolution in Drosophila, including noncoding regions, we have been interested in the contribution of gene regulation to adaptation. Drawing on extensive molecular analysis of the Drosophila even-skipped stripe two enhancer (eS2E) -- arguably the best characterized of any eukaryotic cis-regulatory sequence -- and a rich knowledge of the segmentation process in embryogenesis, Dr. Michael Ludwig (UC) and I have embarked on a research program to functionally dissect the evolution of this enhancer. Our approach has been to exploit transformation technology and the extensive molecular genetic arsenal available for the fly to quantitatively investigate evolved differences in eS2E performance. We are currently investigating features of the eS2E structural architecture that might contribute to functional robustness in enhancer performance, and the evolution of these features. We are also investigating developmental canalization of the segmentation process and the ability of the system to evolve in the face of this canalization. Finally, with the availability of a dozen Drosophila genome sequences, and genome-wide polymorphism data, we are functionally characterizing polymorphic and fixed differences in transcription factor binding sites with the goal of developing novel statistical methods to evaluate mechanisms of selection acting on binding site evolution (see below for a detailed description) <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Ever since our early studies of sequence variation and evolution in Drosophila, including noncoding regions, we have been interested in the contribution of gene regulation to adaptation. Drawing on extensive molecular analysis of the Drosophila even-skipped stripe two enhancer (eS2E) -- arguably the best characterized of any eukaryotic cis-regulatory sequence -- and a rich knowledge of the segmentation process in embryogenesis, Dr. Michael Ludwig (UC) and I have embarked on a research program to functionally dissect the evolution of this enhancer. Our approach has been to exploit transformation technology and the extensive molecular genetic arsenal available for the fly to quantitatively investigate evolved differences in eS2E performance. We are currently investigating features of the eS2E structural architecture that might contribute to functional robustness in enhancer performance, and the evolution of these features. We are also investigating developmental canalization of the segmentation process and the ability of the system to evolve in the face of this canalization. Finally, with the availability of a dozen Drosophila genome sequences, and genome-wide polymorphism data, we are functionally characterizing polymorphic and fixed differences in transcription factor binding sites with the goal of developing novel statistical methods to evaluate mechanisms of selection acting on binding site evolution (see below for a detailed description) <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td></tr>
<!-- diff generator: internal 2013-05-20 17:51:29 -->
</table>Bin Hehttp://www.openwetware.org/index.php?title=Kreitman:Research&diff=499485&oldid=prevBin He: /* Molecular population genetics and evolution (Drosophila and Arabidopsis) */2011-03-17T16:35:30Z<p><span class="autocomment">Molecular population genetics and evolution (Drosophila and Arabidopsis)</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 16:35, 17 March 2011</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Molecular population genetics and evolution (Drosophila and Arabidopsis)==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Molecular population genetics and evolution (Drosophila and Arabidopsis)==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>I have had a longstanding interest in molecular population genetics, and have contributed over the years to the development of methods for analyzing DNA sequence polymorphism and evolutionary data, particularly with respect to detecting the action of natural selection. Although our work has historically focused on Drosophila, we have shifted our attention in recent years to Arabidopsis thaliana and its bacterial pathogens (in collaboration with Joy Bergelson's lab). Plant resistance to bacterial disease and bacterial pathenogenicity is an immensely powerful system for investigating both the ecological and evolutionary contexts of molecular adaptation, and holds the promise of uniting the two. In collaboration with Magnus Nordborg (http://walnut.usc.edu/) and Justin Borevitz, we have completed genome-wide assessments of genetic polymorphism, have assembled at 250K SNP chip, and have genotyped hundreds of isolines derived from natural populations. We have validated genome-wide association mapping in Arabidopsis to discover disease resistance polymorphism, and intend to do the same with common bacterial pathogens. We are also carrying out metagenomic surveys of the bacterial pathogen community infecting Arabidopsis.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>I have had a longstanding interest in molecular population genetics, and have contributed over the years to the development of methods for analyzing DNA sequence polymorphism and evolutionary data, particularly with respect to detecting the action of natural selection. Although our work has historically focused on Drosophila, we have shifted our attention in recent years to Arabidopsis thaliana and its bacterial pathogens (in collaboration with Joy Bergelson's lab). Plant resistance to bacterial disease and bacterial pathenogenicity is an immensely powerful system for investigating both the ecological and evolutionary contexts of molecular adaptation, and holds the promise of uniting the two. In collaboration with Magnus Nordborg (http://walnut.usc.edu/) and Justin Borevitz, we have completed genome-wide assessments of genetic polymorphism, have assembled at 250K SNP chip, and have genotyped hundreds of isolines derived from natural populations. We have validated genome-wide association mapping in Arabidopsis to discover disease resistance polymorphism, and intend to do the same with common bacterial pathogens. We are also carrying out metagenomic surveys of the bacterial pathogen community infecting Arabidopsis.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Ever since our early studies of sequence variation and evolution in Drosophila, including noncoding regions, we have been interested in the contribution of gene regulation to adaptation. Drawing on extensive molecular analysis of the Drosophila even-skipped stripe two enhancer (eS2E) -- arguably the best characterized of any eukaryotic cis-regulatory sequence -- and a rich knowledge of the segmentation process in embryogenesis, Dr. Michael Ludwig (UC) and I have embarked on a research program to functionally dissect the evolution of this enhancer. Our approach has been to exploit transformation technology and the extensive molecular genetic arsenal available for the fly to quantitatively investigate evolved differences in eS2E performance. We are currently investigating features of the eS2E structural architecture that might contribute to functional robustness in enhancer performance, and the evolution of these features. We are also investigating developmental canalization of the segmentation process and the ability of the system to evolve in the face of this canalization. Finally, with the availability of a dozen Drosophila genome sequences, and genome-wide polymorphism data, we are functionally characterizing polymorphic and fixed differences in transcription factor binding sites with the goal of developing novel statistical methods to evaluate mechanisms of selection acting on binding site evolution (see below for a detailed description) <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>Ever since our early studies of sequence variation and evolution in Drosophila, including noncoding regions, we have been interested in the contribution of gene regulation to adaptation. Drawing on extensive molecular analysis of the Drosophila even-skipped stripe two enhancer (eS2E) -- arguably the best characterized of any eukaryotic cis-regulatory sequence -- and a rich knowledge of the segmentation process in embryogenesis, Dr. Michael Ludwig (UC) and I have embarked on a research program to functionally dissect the evolution of this enhancer. Our approach has been to exploit transformation technology and the extensive molecular genetic arsenal available for the fly to quantitatively investigate evolved differences in eS2E performance. We are currently investigating features of the eS2E structural architecture that might contribute to functional robustness in enhancer performance, and the evolution of these features. We are also investigating developmental canalization of the segmentation process and the ability of the system to evolve in the face of this canalization. Finally, with the availability of a dozen Drosophila genome sequences, and genome-wide polymorphism data, we are functionally characterizing polymorphic and fixed differences in transcription factor binding sites with the goal of developing novel statistical methods to evaluate mechanisms of selection acting on binding site evolution (see below for a detailed description) <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Egg size variation==</div></td></tr>
<!-- diff generator: internal 2013-05-20 17:51:29 -->
</table>Bin He