Kristoffer Chin: Week 7
From OpenWetWare
Terms
- Fabs - contains one antigen site for binding and considered a univalent antibody. Has chains linked by disulfide bonds
- Tropism - an involuntary response that can be positive or negative to the source
- Nuclear Magentic Resonance (NMR) - a way of measuring the atomic nuclei in covalent bonds through magnetic moment. NMR uses a spectroscopy type of result
- Syncitia - an epithelium or tissue with cytoplasmic continuity.
- Cyclization - chanign an open chain hydrocarbon into a closed ring
- Glycosylation - process of adding sugar units, addition of glycain chains to proteins. Carbohydrate addition to proteins
- Ramachandran Plot - a graphical representation of the angle of rotation in alpha carbons to carbonyl carbon bonds in polypeptides
- Murine - a member of the rodent family muridae
- Rhinovirus - vrius that infects the upper respiratory tract
- Glycoprotein - protein with covalent attached sugar units bonded by OH or Amine
Outline
- Introduction
- Human immunodeficiency virus type 1 (HIV-1) has an outer surface embedded with spikes made up of envelope glycoproteins gp120 and gp41
- Gp41 responsible for anchoring gp120 to the outer surface
- Gp120 responsible for binding to CD4 receptors
- Gp120 has 5 variable regions
- The V3 region is integral to viral infectivity
- V3 progresses initial infection to full blown AIDS
- Interacts with coreceptors CCR5 or CXCR4
- Called principal neutralizing determinant
- Attracts naturalizing antibodies in the body
- Properties of V3 loops against vaccines
- Different exposure on V3 loop for different strains
- Neutralization resistance
- Inaccessible due to carbohydrate masking and gp120 interactions
- Adapts to different regions on its way to full blown AIDS
- V3 Conformation
- Gp120 adopts several different conformational states
- Variation in V3 region
- Aiming for recognition of antibodies on V3 loop to find a dominant structure
- Previous findings of structures
- V3 structures can be determined through fusion
- V3 insert can adjust its conformation to conform to Fabs 59.1 and 58.2
- V3 conformation to Fabs look similar and predictable through the turns it takes
- Finding a way to stabilize the conformations of V3
- Antibodies used to find the dominant conformation structure for V3 region
BIOL398-01: Bioinformatics Lab
- Lab Journal
- Shared Journal
- Assignments
