Kubke Lab:/Notebook/Cranial nerve development/2011/01/18: Difference between revisions
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* Spoke to Fabiana about the Nissl protocol. She advised that the protocol should be ammended because we are not working with paraffin blocks. The following steps should be used H20--> Crysl Violet-->H20--> 50% Ethanol--> 70/75% Ethanol--> 70% Ethanol with acetic acid( approx 5 drops)-->95% Ethanol-->100% Ethanol 1 --> 100% Ethanol 2--> XYLENE 1,2 and 3. The timing of the Crysl Violet step and differentiation step( 70%eth and acetic acid) should be moderated. --[[User:Malisha Hettiarachchi|Malisha Hettiarachchi]] 19:51, 31 January 2011 (EST) | * Spoke to Fabiana about the Nissl protocol. She advised that the protocol should be ammended because we are not working with paraffin blocks. The following steps should be used H20--> Crysl Violet-->H20--> 50% Ethanol--> 70/75% Ethanol--> 70% Ethanol with acetic acid( approx 5 drops)-->95% Ethanol-->100% Ethanol 1 --> 100% Ethanol 2--> XYLENE 1,2 and 3. The timing of the Crysl Violet step and differentiation step( 70%eth and acetic acid) should be moderated. --[[User:Malisha Hettiarachchi|Malisha Hettiarachchi]] 19:51, 31 January 2011 (EST) | ||
* Feedback form fabiana for [[Kubke_Lab:/Notebook/Cranial_nerve_development/2010/12/10|MH005]] . The warmer temperature works well however neuroepithelium not keeping together. Cut at thickness of 40 microns and 0 degrees( or slightly above 0 degrees) knife angle. | |||
==Reuben== | ==Reuben== |
Revision as of 12:04, 24 February 2011
Cranial Nerve Development | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
General Entries
Personal EntriesMalisha
- Okay I will do this from now on.--Malisha Hettiarachchi 15:01, 23 January 2011 (EST)
Reuben
I did not leave myself enough time to complete the sectioning of embryo RC009. Half the hindbrain and head were discarded and were not captured on the slides.--Reuben Cutfield 22:39, 18 January 2011 (EST) Fabiana
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