Kubke Lab:/Notebook/Cranial nerve development/2011/02/04: Difference between revisions

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* Nissl stain of telencephalon [http://www.frontiersin.org/TempImages/imagecache/77_fnana-01-001/images/image_m/fnana-01-001-g001.jpg] description found [http://www.frontiersin.org/neuroanatomy/10.3389/neuro.05.001.2007/full here]  
* Nissl stain of telencephalon [http://www.frontiersin.org/TempImages/imagecache/77_fnana-01-001/images/image_m/fnana-01-001-g001.jpg] description found [http://www.frontiersin.org/neuroanatomy/10.3389/neuro.05.001.2007/full here]  
*"Mammalia - Felis - Cat spinal cord smear Nissl " [http://biodidac.bio.uottawa.ca/thumbnails/filedet.htm?File_name=19-60B&File_type=GIF]
*"Mammalia - Felis - Cat spinal cord smear Nissl " [http://biodidac.bio.uottawa.ca/thumbnails/filedet.htm?File_name=19-60B&File_type=GIF]
Using the following [http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf resource] previously mentioned [[Kubke_Lab:/Notebook/Cranial_nerve_development/2011/01/28|here]] a set of criteria will be created to assess the quality of the Nissl stained sections:
Using the following [http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf resource] previously mentioned [[Kubke_Lab:/Notebook/Cranial_nerve_development/2011/01/28|here]] a set of criteria will be created to assess the quality of the [[http://openwetware.org/wiki/Nissl_staining|Nissl stained]] sections:
   
   
# Do the cell bodies appear violet to dark blue?
# Do the cell bodies appear violet to dark blue?

Revision as of 19:19, 3 February 2011

Cranial Nerve Development <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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General Entries

  • Insert content here...

Personal Entries

Malisha

Plan for today:

  • Complete entering embryo records including results/staining sections.
  • Complete the required headings in the neuroanatomy document.
  • Follow up embryo atlas

- Nice animation showing 3D embryo development [1]

-Trying to figure out a way to collate the information on google docs so both me and Reuben are able to contribute to the document more collaboratively --Malisha Hettiarachchi 19:55, 3 February 2011 (EST).

Histology

  • A nice video showing coronal sections of mouse brain Nissl stained [2].
  • Nissl stain of mice cerebellum [3]
  • Nissl stain of telencephalon [4] description found here
  • "Mammalia - Felis - Cat spinal cord smear Nissl " [5]

Using the following resource previously mentioned here a set of criteria will be created to assess the quality of the [stained] sections:

  1. Do the cell bodies appear violet to dark blue?
  2. Under higher magnification are subcellular organelles visible?
  3. Is there evidence of cell loss?

Questions to answer:

  • What would the neurons look like in the Nissl stained sections?

The neurons should be stained dark violet against a lighter background colour. The following image illustrates this :[6]

Reuben

  • 10:30am-12:30pm Cresyl violet stain of MH007. The Slides are drying on the benchtop.
  • 1:45pm Working online with Malisha.--Reuben Cutfield 19:53, 3 February 2011 (EST)

Fabiana

  • Enter content here



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