Kubke Lab:Protocols/CVStainTable: Difference between revisions
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|colspan="2"| Date|| | |colspan="2"| Date|| | ||
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|colspan ="3" align=center | '''Defatting and rehydration step''' | |||
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|'''Solution'''||'''Time'''||'''Comments''' | |'''Solution'''||'''Time'''||'''Comments''' | ||
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|Water|| || | |Water|| || | ||
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|colspan ="3" align=center | '''Staining and differentiation step''' | |colspan ="3" align=center | '''Staining and differentiation step''' | ||
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|'''Solution'''||'''Time'''||'''Comments''' | |||
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|Water || || | |Water || || | ||
Latest revision as of 01:03, 27 January 2011
Cresyl Violet staining
For more informattion see Kubke_Lab:Nissl_Stain_Protocol
| Date | ||
| Defatting and rehydration step | ||
| Solution | Time | Comments |
| Water | ||
| 75% alcohol | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Xylene 2 | ||
| Xylene 1 | ||
| 100% alcohol 2 | ||
| 100% alcohol 1 | ||
| 95% alcohol | ||
| 75% alcohol | ||
| Water | ||
| Staining and differentiation step | ||
| Solution | Time | Comments |
| Water | ||
| Cresyl Violet | ||
| 50% alcohol | ||
| 70% alcohol acetic acid | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Coverslip | ||
